Products & ServicesApplications

Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
ResourcesNewsExplore
Oxford Nanopore TechnologiesFully scalable, real-time DNA/RNA sequencing technology
Oxford Nanopore DiagnosticsLamPORE – rapid, low-cost, scalable detection of SARS-CoV-2
Contact
16S Barcoding Kit 1-24
SQK-16S024

Genus-level bacterial identification with barcoding for up to 24 samples.

 Includes a Flow Cell Priming Kit
$900.00

Information

This kit is recommended for users who:

  • want to do 16S sequencing
  • are interested in genus-level bacterial identification

Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail.

Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

No barcodes12 barcodes24 barcodes24 barcodes (Flongle)
Flow cell price$500$500$500$90 (Flongle flow cell)
Library price$90$120$150$150
Price per sample$590$51.67$27.08$10

The 16S Barcoding Kit offers a method of amplifying the entire ~1,500 bp 16S rRNA gene from extracted gDNA, followed by library preparation. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical. On the other hand, analysing the full 16s rRNA gene is more informative than analysing a subset of exons.

The 16S Barcoding Kit features:

FeatureProperty
Preparation time10 minutes + PCR
Input requirement<10 ng gDNA
PCR RequiredYes
FragmentationNot required
Read lengthFull-length 16S gene (~1.5 kb)
Read type produced1D
Typical throughput*★★☆
Associated protocols16S Barcoding Kit 1-24 (SQK-16S024)
Multiplexing options• 16S Barcoding Kit 1-24
• Flow Cell Wash Kit
Pack size6 reactions
StabilityShipped at 2–8°C

Long-term storage -20°C

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters.

The kit is supported by the EPI2ME FASTQ 16S workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

Shipping and logistics:

Flow cells and kits are shipped together at 2–8°C.

Upon receipt, please place the right product in the right long-term storage location.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Workflow

The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 24x barcoded primer pairs.

The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME, which classify the barcode sequence and sort reads into corresponding folders.

16S workflow

Protocols

16S Barcoding Kit 1-24 (SQK-16S024)

To access the protocol, you will need to register for a Nanopore Community account.

What's in the box

The 16S Barcoding Kit 1-24 contains 24 unique barcodes and sufficient reagents to generate six sequencing libraries.

16S kit contents

NameAcronymCap colourNo. of vialsFill volume per vial (μl)
16S Barcode Primers 01-241-24-2 plates, 3 strips per plate15 μl per well
Rapid AdapterRAPGreen110
Sequencing BufferSQBRed1300
Sequencing TetherSQTPurple110
Loading BeadsLBPink1360

The Flow Cell Priming Kit Expansion Pack is also supplied.

FLP

NameAcronymCap colourNo. of vialsFill volume per vial (μl)
Flush BufferFBBlue61,170
Flush TetherFLTPurple1200

3rd party materials

Consumables

  • Agencourt AMPure XP beads
  • 1.5 ml Eppendorf DNA LoBind tubes
  • 0.2 ml thin-walled PCR tubes
  • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
  • Freshly prepared 70% ethanol in nuclease-free water
  • LongAmp HotStart Taq 2X Master Mix (e.g. NEB M0533S)
  • 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

Equipment

  • Microfuge
  • Timer
  • Thermal cycler
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips
  • P2 pipette and tips
  • Multichannel pipette

Optional equipment

  • Agilent Bioanalyzer (or equivalent)
  • Qubit fluorometer (or equivalent for QC check)
  • Eppendorf 5424 centrifuge (or equivalent)

Compatibility

The PCR Sequencing Kit can be used together with:

Kits

  • RAP Top-up Kit (EXP-RAP001)
  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D
  • FLO-FLG001

Devices

  • MinION Mk1B
  • MinION Mk1C
  • GridION
  • Flongle