Information
A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples:
- Midnight Expansion Kit with RT-PCR reagents and primers to amplify SARS-CoV-2 from input RNA
- Rapid Barcoding Kits 96 V14 to barcode and prepare the amplified material for sequencing
- MinION/GridION Flow Cells to perform sequencing on MinION or GridION devices
How it works
The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long). By amplifying longer amplicons, Oxford Nanopores rapid chemistry can be deployed enabling users to rapidly attach a barcode, pool samples and adapt them for sequencing. This method is simple and quick to deploy. It allows rapid turn-around-times that can assist with monitoring of novel variants of concern.
The protocol delivers robust full genome coverage across a wide range of Ct values.
Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow.
The Midnight bundle includes everything you need to sequence SARS-CoV-2:
| Feature | Midnight bundle |
|---|
| Preparation time | 5 h 30 min including RT-PCR |
| Hands-on time | 1 h 15 min |
| Input amount | 8 μl extracted RNA |
| Multiplexing | run in sets of 24* - 96 samples per flow cell |
| Pack size | 576 samples |
| Storage | Shipped at 2-8°C
Flow cells: long-term storage 2-8°C
Kits: long-term storage -20°C |
| Compatibility | MinION Mk1B
MinION Mk1C
GridION Mk1
Please contact us for PromethION |
| 3rd party reagents required | Ethanol, nuclease-free water |
*users wishing to run in multiples of 24 will need to buy 3 x Flow Cell Wash Kits (EXP-WSH004).
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information
Workflow
The sample preparation workflow is simple, flexible and rapid.
Compatibility
The Midnight Bundle can be used together with:
Kits
- Flow Cell Wash Kit (EXP-WSH004)
Flow cells
Devices
- MinION Mk1B
- MinION Mk1C
- GridION Mk1
