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London Calling 2024
  21-24 May
COVID Mini: 576 samples
C19MINI

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

This product is scheduled to be discontinued in March 2024. The recommended replacement is C19MINI114 utilising Q20+ chemistry.

$7,284.00
2Early Access
  • Access the latest products from Oxford Nanopore Technologies
  • Provide feedback into the innovation team as the product develops
  • Product Warranty may initially be limited to 1 month as it is so new
  • Product routinely iterates as new features and capabilities are added
  • Estimated lead time in store
  • Product subject to availability

Information

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples:

  • Midnight Expansion Kit with RT-PCR reagents and primers to amplify SARS-CoV-2 from input RNA
  • Rapid Barcoding Kits 96 to barcode and prepare the amplified material for sequencing
  • MinION/GridION Flow Cells to perform sequencing on MinION or GridION devices
  • Sequence for as little as $12.65 per sample

This product is scheduled to be discontinued in March 2024. The recommended replacement is C19MINI114 utilising Q20+ chemistry.

How it works

The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long). By amplifying longer amplicons, Oxford Nanopores rapid chemistry can be deployed enabling users to rapidly attach a barcode, pool samples and adapt them for sequencing. This method is simple and quick to deploy. It allows rapid turn-around-times that can assist with monitoring of novel variants of concern.

The protocol delivers robust full genome coverage across a wide range of Ct values.

Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow.

image2021-9-8 17-48-8

The Midnight bundle includes everything you need to sequence SARS-CoV-2:

FeatureMidnight bundle
Preparation time5 h 30 min including RT-PCR
Hands-on time1 h 15 min
Input amount8 μl extracted RNA
Multiplexingrun in sets of 24* - 96 samples per flow cell
Pack size576 samples
StorageShipped at 2-8°C

Flow cells: long-term storage 2-8°C

Kits: long-term storage -20°C
CompatibilityMinION Mk1B
MinION Mk1C
GridION Mk1
Please contact us for PromethION
3rd party reagents requiredEthanol, nuclease-free water

*users wishing to run in multiples of 24 will need to buy 3 x Flow Cell Wash Kits (EXP-WSH004).

Shipping and logistics:

Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information

Workflow

The sample preparation workflow is simple, flexible and rapid.

image2021-9-8 17-17-20

What's in the box

This bundle contains:

  • 1 x Midnight Expansion Kit (EXP-MRT001)
  • 2 x Rapid Barcoding Kit 96 (SQK-RBK110.96)
  • 6 x MinION/GridION Flow Cells (FLO-MIN106D)

Compatibility

The Midnight Bundle can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MIN106D

Devices

  • MinION Mk1B
  • MinION Mk1C
  • GridION Mk1