Information
This kit is recommended for users who:
- Are working in the field, with limited access to cold storage and laboratory equipment.
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail.
The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
This product is soon to be discontinued.
This kit is soon to be discontinued and we recommend all customers to upgrade to the latest chemistry for their relevant kit which is available on the Store. If customers require further support for any ongoing critical experiments using a Legacy product, please contact Customer Support via email: support@nanoporetech.com.
The Field Sequencing Kit features:
| Feature | Property |
|---|
| Preparation time | 10 minutes |
| Input requirement | 400 ng gDNA (<30 kb) |
| PCR Required | No |
| Fragmentation | Transposase-based |
| Read length | Random distribution, dependent on input fragment length |
| Kit chemistry | Kit 9 |
| Associated protocols | Rapid sequencing gDNA - Field Sequencing Kit (SQK-LRK001) |
| Multiplexing options | Flow Cell Wash Kit |
| Pack size | 6 reactions |
| Stability | Shipped at 2–8°C
Long-term storage -20°C
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer |
The kit is optimised to be stable at ambient temperatures for extended periods, with many of the reagents supplied in lyophilized form. The stability of the components in this kit enables it to be highly portable, making it ideal for use in the field where access to cold storage may be limited.
Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.
Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.
The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Workflow
The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
Protocols
Rapid sequencing gDNA - Field Sequencing Kit (SQK-LRK001)
To access the protocol, you will need to register for a Nanopore Community account.
What's in the box
The Field Sequencing Kit contains sufficient reagents to generate six sequencing libraries.
| Name | Acronym | Cap colour | No. of vials | Fill volume per vials (μl) |
|---|
| Vial 1: Fragmentation Mix | FRL | Clear | 6 | lyophilised reagent |
| Vial 2: Rapid Adapter | RPL | Clear | 6 | lyophilised reagent |
| Vial 3: Sequencing Buffer | SBL | Clear | 6 | lyophilised reagent |
| Resuspension Buffer | RTB | Pink | 1 | 500 |
The Flow Cell Priming Kit is also supplied.
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (μl) |
|---|
| Flush Buffer | FB | Blue | 6 | 1,170 |
| Flush Tether | FLT | Purple | 1 | 200 |
Compatibility
The Field Sequencing Kit can be used together with:
Kits
- Flow Cell Wash Kit (EXP-WSH004)
Flow cells
Devices
- MinION Mk1B
- MinION Mk1C
- GridION