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Field Sequencing Kit
SQK-LRK001

Cold chain-free library preparation for ultimate portability.

 Includes a Flow Cell Priming Kit
$599.00

Information

This kit is recommended for users who:

  • are working in the field, with limited access to cold storage and laboratory equipment.

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

The Field Sequencing Kit features:

FeatureProperty
Preparation time10 minutes
Input requirement400 ng of high molecular weight gDNA (<30 kb)
PCR RequiredNo
FragmentationTransposase-based
Read lengthRandom distribution, dependent on input fragment length
Read type produced1D
Typical throughput*★★☆
Associated protocolsField Sequencing Kit
Multiplexing optionsFlow Cell Wash Kit
Pack size6 reactions
StabilityShipped at 2–8°C

Long-term storage: up to 30°C for one month unopened, or at 2–8°C for three months unopened.

The Flow Cell Priming Kit shipped with the Field Sequencing Kit can be stored in the same conditions.

The kit is optimised to be stable at ambient temperatures for extended periods, with many of the reagents supplied in lyophilized form. The stability of the components in this kit enables it to be highly portable, making it ideal for use in the field where access to cold storage may be limited.

LRK001-read-length

Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.

LRK001-throughput Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.

The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.

Shipping and logistics:

Flow cells and kits are shipped together at 2–8°C.

Upon receipt, please place the right product in the right long-term storage location.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Workflow

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

RAD004 v1

Protocols

Field Sequencing Kit

To access the protocol, you will need to register for a Nanopore Community account.

What's in the box

The Field Sequencing Kit contains sufficient reagents to generate six sequencing libraries. Field Sequencing Kit contents

NameAcronymCap colourNo. of vialsFill volume per vials (μl)
Vial 1: Fragmentation MixFRLClear6lyophilised reagent
Vial 2: Rapid AdapterRPLClear6lyophilised reagent
Vial 3: Sequencing BufferSBLClear6lyophilised reagent
Resuspension BufferRTBPink1500

The Flow Cell Priming Kit is also supplied.

FLP

NameAcronymCap colourNo. of vialsFill volume per vial (μl)
Flush BufferFBBlue61,170
Flush TetherFLTPurple1200

3rd party materials

Equipment

  • Method of achieving 80°C
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips

Compatibility

The Field Sequencing Kit can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • MinION Mk1B
  • MinION Mk1C
  • GridION