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Oxford Nanopore TechnologiesFully scalable, real-time DNA/RNA sequencing technology
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London Calling 2022
18th - 20th May
PromethION Flow Cell Packs (R10.4)
FLO-PRO112

R10 is our nanopore chemistry designed to deliver highest consensus accuracy. Paired with the Kit 12 (also referred to as Q20+) chemistry, R10.4 generates data at a modal accuracy above 99%.

As a new product, it will be rapidly iterating as we continue to deliver performance improvements.

This is an Early Access product

Note: R10.4 flow cells currently require Kit 12 chemistry.

It is currently supported for the Ligation Sequencing Kit range, however, further kit compatibility will be added.

A pack contains 4 PromethION flow cells

Product lead time: 2 weeks

Chemistry type:
R10.4
Pack type:
Annual packs
Pack size:
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From:
$5,600.00
2Early Access
  • Access the latest products from Oxford Nanopore Technologies
  • Provide feedback into the innovation team as the product develops
  • Product Warranty may initially be limited to 1 month as it is so new
  • Product routinely iterates as new features and capabilities are added
  • Estimated lead time in store
  • Product subject to availability

Information

A PromethION Flow Cell (R10.4) is a consumable used with PromethION P24 and P48 devices. It contains the proprietary sensor array, Application-Specific Integrated Circuit (ASIC), and R10 nanopores. The R10 series of nanopores contains a double reader-head, and is suitable for experiments where high consensus accuracy is required. Paired with the Kit 12 (also referred to as Q20+) chemistry, R10.4 generates data at a modal accuracy above 99%. As a new product, it will be rapidly iterating.

The initial release supports the ligation sequencing chemistry, with other sequencing kit support currently in development.

How it works:

A user simply adds their prepared library to the flow cell and starts their sequencing experiment.

Data is accumulated over time as DNA or RNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.

As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~72 hours (under optimal conditions) and a user can choose to run continuously or run, stop, wash, and load a new sample until the buffer and nanopores are exhausted.

Shipping and logistics:

PromethION Flow Cells are packaged in packs of 4. The flow cells are shipped in multiple set sizes, depending on the size of your project. All orders can be split for delivery to best suit your needs.

Flow cells and kits are shipped together at 2–8°C. The packaging is designed to protect the flow cells from freezing. Flow cells and sequencing kits are stable at room temperature for up to seven days, so users should not be concerned about their products should they arrive at ambient temperature.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Storage and stability:

Please store your flow cells as follows:

  • Room temperature: during Early Access we ask users to store their flow cells at 2-8°C. Flow cells are stable for a few days at room temperature, however we will formalise guidance during the course of product roll-out.
  • 2-8°C: flow cells can be stored, unopened, at 2-8°C for 4 weeks.

We recommend you check your flow cell before preparing your DNA/RNA library as part of the warranty process.

Flow cell returns:

We ask customers to return their used flow cells to Oxford Nanopore Technologies for recycling. For more information, please see the "Flow cell returns" section.

Workflow

Prior to use, a Flow Cell Check should be run on the flow cell. This checks the environment for the flow cell chemistry has been maintained during shipping.

PromethION Flow Cells are provided in a sealed pouch and are shipped at a constant temperature. For long-term storage, it is recommended that they are kept in an environment not prone to large temperature fluctuations. The flow cells should be stored flat.

Once the Flow Cell Check has been performed satisfactorily, the flow cell is ready to be used for experiments. The exact protocol and reagent kits used will depend on the experiment being run.

Please return your flow cells to Oxford Nanopore Technologies after use.

Safety and legal

Safety Data Sheet for PromethION Flow Cell buffer

Customers are required to perform a flow cell check within 1 month of purchase. Oxford Nanopore Technologies will replace any flow cell with fewer than 5000 nanopores, when the result is reported within two days of performing the flow cell check, and when the storage recommendations have been followed. We deem the useful life of this product to be 4 weeks from receipt by the customer.

There is a 1 year contract length on all consumables purchases, enabling you to schedule your shipments through this time frame.

What's in the box

PromethION flow cells are shipped in a sealed pouch in packs of four.

FLO-PRO002

Multiplexing

The Flow Cell Wash Kit allows sequential runs of multiple sequencing libraries in the same flow cell. It works by washing out the first library, and refreshing the system ready for a subsequent library to be loaded. This procedure provides the opportunity to utilise the same flow cell a number of times, maximising the available run time particularly for cases where less data per library is required. Following the wash step, Storage Buffer is introduced into the flow cell, allowing storage of the flow cell before subsequent library addition.

Compatibility

Kits

  • Ligation Sequencing Kit (SQK-LSK112)
  • Ligation Sequencing Kit XL (SQK-LSK112-XL)
  • Native Barcoding Kit 24 (SQK-NBD112.24)
  • Native Barcoding Kit 96 (SQK-NBD112.96)