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Rapid Barcoding Kit 96
SQK-RBK110.96

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples

This product has a one-week lead time

$990.00

Information

This kit is recommended for users who:

  • wish to multiplex samples to reduce price per sample
  • need a PCR-free method of multiplexing to preserve additional information such as base modifications
  • require a short preparation time
  • have limited access to laboratory equipment

Please note that to use this kit, you will need to purchase additional 3rd party materials: see the "3rd Party Materials" tab for more detail.

Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

No barcodes6 barcodes12 barcodes96 barcodes
Flow cell price$500$500$500$500
Library price$99$99$99$99
Price per sample$599$104$54$5

The Rapid Barcoding Kit 96 features:

FeatureProperty
Preparation time60 minutes
Input requirement50 ng of high molecular weight gDNA (>30 kb)
PCR RequiredNo
FragmentationTransposase-based
Read lengthRandom distribution, dependent on input fragment length
Read type produced1D
Typical throughput*★★☆
Associated protocolsRapid Barcoding Kit 96
PCR tiling of SARS-CoV-2 virus with rapid barcoding
Multiplexing options• Rapid Barcoding Kit 96
• Flow Cell Wash Kit
Pack size3 x 96 reactions with reagents to support 12 x 24 reactions
StabilityShipped at 2–8° C

Long-term storage –20°C

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

The kit is optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required, some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.

The workflow is PCR-free and therefore removes PCR bias and retains base modification information which can be analysed using tools developed in the Nanopore Community.

Deconvolution of barcoded sequencing data is supported by MinKNOW and Guppy software, which classify the barcode sequence and sort reads into corresponding folders.

Further considerations:

The Rapid Barcoding Kit recommends an input of 50 ng gDNA per sample, and is optimised for samples which contain long fragments (>30 kb). Addition of less than 50 ng, or shorter fragments could compromise sequencing throughput and read length. For samples where only lower inputs are possible, the Rapid PCR Barcoding Kit is available.

Shipping and logistics:

Flow cells and kits are shipped together at 2–8°C.

Upon receipt, please place the right product in the right long-term storage location.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Workflow

The Rapid Barcoding Kit 96 generates barcoded sequencing libraries from extracted gDNA in 60 minutes using a simple protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches barcoded tags to the cleaved ends: there are 96 unique barcoded tags in the kit. Barcoded samples are pooled, purified using SPRI beads, and Rapid Sequencing Adapters are then added to the tagged ends.

SQK-RBK110.96 gDNA workflow v1

Protocols

Rapid Barcoding Kit 96PCR tiling of SARS-CoV-2 virus with rapid barcoding

To access the protocols, you will need to register for a Nanopore Community account.

What's in the box

SQK-RBK110.96 kit contents v1

NameAcronymCap colourNo. of vialsFill volume per vial (µl)
Rapid Barcode plateRB96-3 plates8 µl per well
SPRI Beads*SPRIBrown31,200
Sequencing Buffer IISBIIRed1500
Rapid Adapter FRAP-FGreen125
Elution BufferEBBlack1500
Loading Beads IILBIIPink1360
Loading SolutionLSWhite cap, pink label1400
Flush TetherFLTPurple1400
Flush BufferFBWhite1 bottle15,500

* This product contains AMPure XP reagent manufactured by Beckman Coulter, Inc.

3rd party materials

Consumables

  • 1.5 ml Eppendorf DNA LoBind tubes
  • 2 ml Eppendorf DNA LoBind tubes
  • 0.2 ml thin-walled PCR tubes
  • Eppendorf twin.tec® PCR plate 96 LoBind, semi-skirted (Cat # 0030129504) with heat seals
  • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
  • Freshly-prepared 80% ethanol in nuclease-free water

Equipment

  • Ice bucket with ice
  • Microplate centrifuge, e.g. Fisherbrand™ Mini Plate Spinner Centrifuge (Fisher Scientific, #11766427)
  • Timer
  • Thermal cycler or heat block at 30°C and 80°C
  • Magnetic rack
  • Hula mixer (gentle rotator mixer)
  • P1000 pipette and tips
  • P200 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P2 pipette and tips
  • Multichannel pipette

Optional equipment

  • Qubit fluorometer (or equivalent for QC check)

Compatibility

The Rapid Barcoding Kit 96 can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D
  • FLO-MIN111
  • FLO-PRO002
  • FLO-FLG001

Devices

  • MinION Mk1B
  • MinION Mk1C
  • Flongle
  • GridION
  • PromethION