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Rapid Sequencing Kit
SQK-RAD004

A simple and rapid library preparation method for genomic DNA.

 Includes a Flow Cell Priming Kit
$599.00

Information

This kit is recommended for users who:

  • require a short preparation time
  • have limited access to laboratory equipment

The Rapid Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

The Rapid Sequencing Kit features:

FeatureProperty
Preparation time10 minutes
Input requirement400 ng of high molecular weight gDNA (<30 kb)
PCR RequiredNo
FragmentationTransposase-based
Read lengthRandom distribution, dependent on input fragment length
Read type produced1D
Typical throughput★★☆
Associated protocolsRapid Sequencing
Rapid Lambda Control Experiment
Rapid whole genome amplification
Multiplexing options• Rapid Barcoding Kit
• Flow Cell Wash Kit
Pack size6 reactions
StabilityShipped at 2–8°C

Long-term storage -20°C

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

The kit is optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required (e.g. minimal pipetting steps and no clean-ups) some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.

RAD004 v1

Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.

2017 07 17 RAD003 TP v6 DS-01

Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.

The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.

Further considerations:

The Rapid Sequencing Kit recommends an input of 400 ng gDNA, and is optimised for samples which contain long fragments (>30 kb). Addition of less than 400 ng, or the presence of shorter fragments could compromise sequencing throughput and read length. For samples where only lower inputs are possible, the Low Input by PCR Sequencing Kit and Rapid Low Input by PCR Sequencing Kit are available.

Compare Sequencing Kits

Shipping and logistics:

Flow cells and kits are shipped together at 2–8°C.

Upon receipt, please place the right product in the right long-term storage location.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Workflow

The Rapid Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

RAD004 v1

Protocols

Rapid Sequencing
Rapid Lambda Control Experiment
Rapid whole genome amplification

To access the protocols, you will need to register for a Nanopore Community account.

What's in the box

The Rapid Sequencing Kit contains sufficient reagents to generate six sequencing libraries.

2017 11 16 RAD004 v2 DS

NameAcronymCap colourNo. of vialsFill volume per vial (µl)
Lambda DNALMDYellow188
Fragmentation MixFRABrown130
Rapid AdapterRAPGreen110
Sequencing TetherSQTPurple110
Loading BeadsLBPink1360
Sequencing BufferSQBRed1300

The Flow Cell Priming Kit is also supplied.

FLP

NameAcronymCap colourNo. of vialsFill volume per vial (μl)
Flush BufferFBBlue61,170
Flush TetherFLTPurple1200

3rd party materials

Consumables

  • 1.5 ml Eppendorf DNA LoBind tubes
  • 0.2 ml thin-walled PCR tubes
  • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)

Equipment

  • Microfuge
  • Vortex mixer
  • Thermal cycler at 30°C and 80°C
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips
  • P2 pipette and tips
  • Timer

Optional equipment

  • Qubit fluorometer (or equivalent for QC check)

Additional materials for:

Rapid whole genome amplification

  • Qiagen REPLI-g UltraFast Mini Kit
  • Phosphate buffered saline (PBS)

Compatibility

The Rapid Sequencing Kit can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D
  • FLO-PRO002

Devices

  • MinION Mk1B
  • MinION Mk1C
  • GridION
  • PromethION