Information
This kit is recommended for users who:
- Require a short preparation time
- Have limited access to laboratory equipment
This is an Early Access product.
For more information about our Early Access programmes, please see this article on product release phases.
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail.
The Rapid Sequencing Kit V14 features:
The Rapid Sequencing Kit V14 (SQK-RAD114) is a standalone kit optimised for speed and simplicity to sequence libraries using a PCR-free method. The kit uses a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends for sequencing on a flow cell.
This kit has been updated to use our newest Kit 14 chemistry which includes improved modal raw read sequencing accuracies with higher output on our latest nanopore: R10.4.1. The flow cell priming and sequencing reagents are now included as part of the sequencing kit and have been reformulated to be compatible with the chemistry upgrade and R10.4.1 nanopore.
Kit 14 also includes previous updates such as the higher capture rate of DNA to enable lower flow cell loading amounts, and fuel fix technology, allowing users to run longer experiments without the need for fuel addition during the run.
Note: This kit is able to generate high sequencing accuracies of over 99% (Q20+) and duplex data; but has been optimised for speed and simplicity. If users require high accuracy, we recommend using our Ligation Sequencing Kit V14 (SQK-LSK114).
Due to the simple nature of the workflow and the fact that little sample manipulation is required (e.g. minimal pipetting steps and no clean-up steps) some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.
The workflow is PCR-free, removing the PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.
Further considerations:
The Rapid Sequencing Kit recommends an input of 100 ng gDNA, and is optimised for samples which contain long fragments (>30 kb). Addition of less than 100 ng, or the presence of shorter fragments could compromise sequencing output and read length.
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Workflow
The Rapid Sequencing Kit V14 generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
What's in the box
The Rapid Sequencing Kit V14 (SQK-RAD114) contains sufficient reagents for six reactions.
Note: We are in the process of reformatting our kits with single-use tubes into a bottle format.
Single-use tubes format:
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (µl) |
|---|
| Fragmentation Mix | FRA | Orange | 1 | 10 |
| Rapid Adapter | RA | Green | 1 | 15 |
| Adapter Buffer | ADB | Clear | 1 | 100 |
| Library Beads | LIB | Pink | 1 | 600 |
| Library Solution | LIS | White cap, pink label | 1 | 600 |
| Sequencing Buffer | SB | Red | 1 | 700 |
| Flow Cell Flush | FCF | Blue | 6 | 1170 |
| Flow Cell Tether | FCT | Purple | 1 | 200 |
Bottle format:
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (µl) |
|---|
| Fragmentation Mix | FRA | Orange | 1 | 10 |
| Rapid Adapter | RA | Green | 1 | 15 |
| Adapter Buffer | ADB | Clear | 1 | 100 |
| Library Beads | LIB | Pink | 1 | 600 |
| Library Solution | LIS | White cap, pink label | 1 | 600 |
| Sequencing Buffer | SB | Red | 1 | 700 |
| Flow Cell Flush | FCF | Clear cap, light blue label | 1 | 8,000 |
| Flow Cell Tether | FCT | Purple | 1 | 200 |
Compatibility
The Rapid Sequencing Kit V14 can be used together with:
Kits:
- Flow Cell Wash Kit (EXP-WSH004)
- Control Expansion (EXP-CTL001)
- Sequencing Auxiliary Expansion V14 (EXP-AUX003)
- Flow Cell Priming Kit (EXP-FLP004)
- Flow Cell Priming Kit XL (EXP-FLP004-XL)
- Rapid Adapter Auxiliary V14 (EXP-RAA114)
Flow cells:
R10.4.1:
- FLO-MIN114
- FLO-PRO114M
- FLO-FLG114
Devices:
- Flongle
- MinION Mk1B
- MinION Mk1C
- GridION
- PromethION