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VolTRAX Sequencing Kit

VSK-VSK002

$149.00

A simple and rapid library preparation method for genomic DNA on the VolTRAX

Includes a Flow Cell Priming Kit

gDNA 10 mins No PCR Automation
  • Information

    This kit is recommended for users who

    • require a short preparation time
    • would like to do automated library preparation on the VolTRAX

     

    The VolTRAX Sequencing Kit generates sequencing libraries from extracted gDNA in 5-10 minutes hands-on time plus 45 minutes automation. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Sequencing Adapters are then added to the tagged ends, and the library is cleaned up using SPRI beads.

    The VolTRAX Sequencing Kit features:

    FeatureProperty
    Preparation time 5-10 minutes hands-on time, 45 mins automation
    Input requirement 475 ng gDNA
    PCR Required No
    Fragmentation Transposase-based
    Read length Random distribution, dependent on input fragment length
    Read type produced 1D
    Typical throughput* •••
    Associated protocols

    VolTRAX protocol

    Pack size 3 reactions
    Stability

    Shipped at  2-8° C

    Long-term storage  -20° C

     

    The kit is optimised for simplicity and speed, rather than obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required, some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.

  • Safety and legal
  • What's in the box

    The VolTRAX Sequencing Kit contains sufficient reagents to generate three sequencing libraries.

    The Flow Cell Priming Kit (EXP-FLP002) is also supplied.

  • Compatibility

    The VolTRAX Sequencing Kit can be used together with:

    Kits

    • Flow Cell Wash Kit (EXP-WSH003)

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D

    Devices

    • VolTRAX V2

    The library prepared on the VolTRAX can be sequenced on:

    • MinION Mk 1B
    • GridION X5
Information

This kit is recommended for users who

  • require a short preparation time
  • would like to do automated library preparation on the VolTRAX

 

The VolTRAX Sequencing Kit generates sequencing libraries from extracted gDNA in 5-10 minutes hands-on time plus 45 minutes automation. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Sequencing Adapters are then added to the tagged ends, and the library is cleaned up using SPRI beads.

The VolTRAX Sequencing Kit features:

FeatureProperty
Preparation time 5-10 minutes hands-on time, 45 mins automation
Input requirement 475 ng gDNA
PCR Required No
Fragmentation Transposase-based
Read length Random distribution, dependent on input fragment length
Read type produced 1D
Typical throughput* •••
Associated protocols

VolTRAX protocol

Pack size 3 reactions
Stability

Shipped at  2-8° C

Long-term storage  -20° C

 

The kit is optimised for simplicity and speed, rather than obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required, some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.

What's in the box

The VolTRAX Sequencing Kit contains sufficient reagents to generate three sequencing libraries.

The Flow Cell Priming Kit (EXP-FLP002) is also supplied.

Compatibility

The VolTRAX Sequencing Kit can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH003)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • VolTRAX V2

The library prepared on the VolTRAX can be sequenced on:

  • MinION Mk 1B
  • GridION X5
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