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Field Sequencing Kit

SQK-LRK001

$599.00

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

gDNA 400 ng 10 mins No PCR Cold Chain-Free
  • Information

    This kit is recommended for users who:

    • are working in the field, with limited access to cold storage and laboratory equipment.
    The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
     

    The Field Sequencing Kit features:

    Feature Property
    Preparation time 10 minutes
    Input requirement 400 ng of high molecular weight gDNA (>30 kb)
    PCR Required No
    Fragmentation Transposase-based
    Read length Random distribution, dependent on input fragment length
    Read type produced 1D
    Typical throughput* ••
    Associated protocols

    Field Sequencing Kit

    Multiplexing options

    Flow Cell Wash Kit

    Pack size 6 reactions
    Stability

    Shipped at  2–8° C

    Long-term storage: up to 30° C for one month unopened, or at 2–8° C for three months unopened

    The Flow Cell Priming Kit shipped with the Field Sequencing Kit can be stored in the same conditions.

     

    The kit is optimised to be stable at ambient temperatures for extended periods, with many of the reagents supplied in lyophilized form. The stability of the components in this kit enables it to be highly portable, making it ideal for use in the field where access to cold storage may be limited.

     

     

    Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.

     

    Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.

    The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.

    Further considerations:

    Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

     

  • Workflow

    The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

  • Protocols
  • Safety and legal
  • What's in the box

    The Field Sequencing Kit contains sufficient reagents to generate six sequencing libraries.

     

    The Flow Cell Priming Kit is also supplied.

    EXP-FLP002

  • 3rd party materials

    Equipment

    • Method of achieving 80° C
    • P1000 pipette and tips
    • P100 pipette and tips
    • P20 pipette and tips
    • P10 pipette and tips
  • Compatibility

    The Field Sequencing Kit can be used together with:

    Kits

    • Flow Cell Wash Kit (EXP-WSH003)

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D

    Devices

    • MinION Mk 1B
    • GridION X5
Information

This kit is recommended for users who:

  • are working in the field, with limited access to cold storage and laboratory equipment.
The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
 

The Field Sequencing Kit features:

Feature Property
Preparation time 10 minutes
Input requirement 400 ng of high molecular weight gDNA (>30 kb)
PCR Required No
Fragmentation Transposase-based
Read length Random distribution, dependent on input fragment length
Read type produced 1D
Typical throughput* ••
Associated protocols

Field Sequencing Kit

Multiplexing options

Flow Cell Wash Kit

Pack size 6 reactions
Stability

Shipped at  2–8° C

Long-term storage: up to 30° C for one month unopened, or at 2–8° C for three months unopened

The Flow Cell Priming Kit shipped with the Field Sequencing Kit can be stored in the same conditions.

 

The kit is optimised to be stable at ambient temperatures for extended periods, with many of the reagents supplied in lyophilized form. The stability of the components in this kit enables it to be highly portable, making it ideal for use in the field where access to cold storage may be limited.

 

 

Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.

 

Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.

The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.

Further considerations:

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

 

Workflow

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

Protocols
What's in the box

The Field Sequencing Kit contains sufficient reagents to generate six sequencing libraries.

 

The Flow Cell Priming Kit is also supplied.

EXP-FLP002

3rd party materials

Equipment

  • Method of achieving 80° C
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips
Compatibility

The Field Sequencing Kit can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH003)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • MinION Mk 1B
  • GridION X5
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