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16S Barcoding Kit 1-24

SQK-16S024

$900.00

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit
Product has a lead time of 6 weeks

gDNA 10 ng ~10 mins + PCR PCR Bacterial ID
  • Information

    This kit is recommended for users who:

    • want to do 16S sequencing
    • are interested in genus-level bacterial identification

    Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

    Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

     

     

    No barcodes

     12 barcodes

     24 barcodes

     24 barcodes (Flongle)

    Flow cell price

    $500

     $500

      $500

     $90 (Flongle flow cell)

    Library price

    $90

     $120

     $150

     $150

    Price per sample

    $590

     $51.67

     $27.08

     $10

     

    The 16S Barcoding Kit offers a method of amplifying the entire ~1,500 bp 16S rRNA gene from extracted gDNA, followed by library preparation. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical. On the other hand, analysing the full 16s rRNA gene is more informative than analysing a subset of exons.

    The 16S Barcoding Kit features:

    Feature Property
    Preparation time 10 minutes + PCR
    Input requirement <10 ng gDNA
    PCR Required Yes
    Fragmentation Not required
    Read length Full-length 16S gene (~1.5 kb)
    Read type produced 1D
    Typical throughput* ••
    Associated protocols

    16S Barcoding Kit 1-24 (SQK-16S024)

    Multiplexing options

    16S Barcoding Kit 1-24

    Wash Kit

    Pack size 6 reactions
    Stability

    Stable at RT for 7 days

    Shipped at  2-8° C

    Long-term storage  -20° C

     

    The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters.

    The kit is supported by the EPI2ME FASTQ 16S workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

    Further considerations:

    Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

  • Workflow

    The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 24x barcoded primer pairs.

    The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME, which classify the barcode sequence and sort reads into corresponding folders.

  • Protocols
  • Safety and legal
  • What's in the box

    The 16S Barcoding Kit 1-24 contains 24 unique barcodes and sufficient reagents to generate six sequencing libraries.

     

    The Flow Cell Priming Kit Expansion Pack is also supplied.

    EXP-FLP002

  • 3rd party materials

    Consumables

    • Agencourt AMPure XP beads
    • 1.5 ml Eppendorf DNA LoBind tubes
    • 0.2 ml thin-walled PCR tubes
    • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
    • Freshly prepared 70% ethanol in nuclease-free water
    • LongAmp HotStart Taq 2X Master Mix (e.g. NEB M0533S)
    • 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

    Equipment

    • Microfuge
    • Timer
    • Thermal cycler
    • P1000 pipette and tips
    • P100 pipette and tips
    • P20 pipette and tips
    • P10 pipette and tips
    • P2 pipette and tips
    • Multichannel pipette

    Optional Equipment

    • Agilent Bioanalyzer (or equivalent)
    • Qubit fluorometer (or equivalent for QC check)
    • Eppendorf 5424 centrifuge (or equivalent)
  • Compatibility

    The PCR Sequencing Kit can be used together with:

    Kits

    • RAP Top-up Kit (EXP-RAP001)
    • Flow Cell Wash Kit (EXP-WSH003)

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D
    • FLO-FLG001

    Devices

    • MinION Mk 1B
    • MinION Mk 1C
    • GridION Mk1
    • Flongle
Information

This kit is recommended for users who:

  • want to do 16S sequencing
  • are interested in genus-level bacterial identification

Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

 

 

No barcodes

 12 barcodes

 24 barcodes

 24 barcodes (Flongle)

Flow cell price

$500

 $500

  $500

 $90 (Flongle flow cell)

Library price

$90

 $120

 $150

 $150

Price per sample

$590

 $51.67

 $27.08

 $10

 

The 16S Barcoding Kit offers a method of amplifying the entire ~1,500 bp 16S rRNA gene from extracted gDNA, followed by library preparation. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical. On the other hand, analysing the full 16s rRNA gene is more informative than analysing a subset of exons.

The 16S Barcoding Kit features:

Feature Property
Preparation time 10 minutes + PCR
Input requirement <10 ng gDNA
PCR Required Yes
Fragmentation Not required
Read length Full-length 16S gene (~1.5 kb)
Read type produced 1D
Typical throughput* ••
Associated protocols

16S Barcoding Kit 1-24 (SQK-16S024)

Multiplexing options

16S Barcoding Kit 1-24

Wash Kit

Pack size 6 reactions
Stability

Stable at RT for 7 days

Shipped at  2-8° C

Long-term storage  -20° C

 

The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters.

The kit is supported by the EPI2ME FASTQ 16S workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

Further considerations:

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Workflow

The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 24x barcoded primer pairs.

The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME, which classify the barcode sequence and sort reads into corresponding folders.

What's in the box

The 16S Barcoding Kit 1-24 contains 24 unique barcodes and sufficient reagents to generate six sequencing libraries.

 

The Flow Cell Priming Kit Expansion Pack is also supplied.

EXP-FLP002

3rd party materials

Consumables

  • Agencourt AMPure XP beads
  • 1.5 ml Eppendorf DNA LoBind tubes
  • 0.2 ml thin-walled PCR tubes
  • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
  • Freshly prepared 70% ethanol in nuclease-free water
  • LongAmp HotStart Taq 2X Master Mix (e.g. NEB M0533S)
  • 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

Equipment

  • Microfuge
  • Timer
  • Thermal cycler
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips
  • P2 pipette and tips
  • Multichannel pipette

Optional Equipment

  • Agilent Bioanalyzer (or equivalent)
  • Qubit fluorometer (or equivalent for QC check)
  • Eppendorf 5424 centrifuge (or equivalent)
Compatibility

The PCR Sequencing Kit can be used together with:

Kits

  • RAP Top-up Kit (EXP-RAP001)
  • Flow Cell Wash Kit (EXP-WSH003)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D
  • FLO-FLG001

Devices

  • MinION Mk 1B
  • MinION Mk 1C
  • GridION Mk1
  • Flongle
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