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Rapid Barcoding Kit 96

SQK-RBK110.96

$990.00

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples
This product has a 1 week lead time

  • Information

    Rapid Barcoding Kit 96

    This kit is recommended for users who:

    • wish to multiplex samples to reduce price per sample
    • need a PCR-free method of multiplexing to preserve additional information such as base modifications
    • require a short preparation time
    • have limited access to laboratory equipment.

    Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

    Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

     No barcodes6 barcodes12 barcodes96 barcdoes

    Flow cell price

    $500

    $500

    $500

    $500

    Library price

    $99

    $99

    $99

    $99

    Price per sample

    $599

    $104

    $54

    $5

     

    Feature

    Property

    Preparation time

    60 minutes

    Input requirement

    50 ng of high molecular weight gDNA (>30 kb)

    PCR Required

    No

    Fragmentation

    Transposase-based

    Read length

    Random distribution, dependent on input fragment length

    Read type produced

    1D

    Typical throughput*

    ••

    Associated protocols

    Rapid Barcoding Kit 96 (SQK-RBK110.96)

    PCR tiling of SARS-CoV-2 virus with rapid barcoding (SQK-RBK110.96)

    Multiplexing options

    Rapid Barcoding Kit

    Flow Cell Wash Kit

    Pack size

    3 x 96 reactions with reagents to support 12 x 24 reactions

    Stability

    Shipped at  2–8° C

    Long-term storage  -20° C

    Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

    The kit is optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required, some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.

    The workflow is PCR-free and therefore removes PCR bias and retains base modification information which can be analysed using tools developed in the Nanopore Community.

    Deconvolution of barcoded sequencing data is supported by MinKNOW and Guppy software, which classify the barcode sequence and sort reads into corresponding folders.

    Further considerations:

    The Rapid Barcoding Kit recommends an input of 50 ng gDNA per sample, and is optimised for samples which contain long fragments (>30 kb). Addition of less than 50 ng, or shorter fragments could compromise sequencing throughput and read length. For samples where only lower inputs are possible, the Rapid PCR Barcoding Kit is available.

    Shipping and logistics:

    Flow cells and kits are shipped together at 2–8°C.

    Upon receipt, please place the right product in the right long-term storage location.

    Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

    The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

  • Workflow

    The Rapid Barcoding Kit 96 generates barcoded sequencing libraries from extracted gDNA in 60 minutes using a simple protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches barcoded tags to the cleaved ends: there are 12 unique barcoded tags in the kit. Barcoded samples are pooled, purified using SPRI beads, and Rapid Sequencing Adapters are then added to the tagged ends. 

    SQK-RBK110.96_gDNA_workflow_v1

  • Protocols
  • Safety and legal
  • What's in the box

    SQK-RBK110.96_kit_contents_v1

    Name

    Acronym

    Cap colour

    No. of tubes

    Fill volume (μl)

    Rapid Barcode plate

    RB96

    -

    3 plates

    2.5 μl per well

    SPRI Beads

    SPRI

    Brown

    3

    960

    Sequencing Buffer II

    SBII

    Red

    1

    408

    Rapid Adapter F

    RAP-F

    Green

    1

    15

    Loading Beads II

    LBII

    Pink

    1

    306

    Elution Buffer

    EB

    Black

    1

    420

    Loading Solution

    LS

    White cap, pink label

    1

    306

    Flush Tether

    FLT

    Purple

    1

    360

    Flush Buffer

    FB

    White

    1 bottle

    14,040

  • 3rd party materials

    Consumables

    • 1.5 ml Eppendorf DNA LoBind tubes
    • 2 ml Eppendorf DNA LoBind tubes
    • 0.2 ml thin-walled PCR tubes
    • Eppendorf twin.tec® PCR plate 96 LoBind, semi-skirted (Cat # 0030129504) with heat seals
    • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
    • Freshly-prepared 80% ethanol in nuclease-free water

    Equipment

    • Ice bucket with ice
    • Microplate centrifuge, e.g. Fisherbrand™ Mini Plate Spinner Centrifuge (Fisher Scientific, #11766427)
    • Timer
    • Thermal cycler or heat block at 30°C and 80°C
    • Magnetic rack
    • Hula mixer (gentle rotator mixer)
    • P1000 pipette and tips
    • P200 pipette and tips
    • P100 pipette and tips
    • P20 pipette and tips
    • P2 pipette and tips
    • Multichannel pipette

    Optional Equipment

    • Qubit fluorometer (or equivalent for QC check)
  • Compatibility

    The Rapid Barcoding Kit 96 can be used together with:

    Kits

    • Flow Cell Wash Kit (EXP-WSH004)

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D
    • FLO-MIN111
    • FLO-PRO002
    • FLO-FLG001

    Devices

    • MinION Mk1B
    • MinION Mk1C
    • GridION Mk1
    • PromethION P24/48
    • Flongle
Information

Rapid Barcoding Kit 96

This kit is recommended for users who:

  • wish to multiplex samples to reduce price per sample
  • need a PCR-free method of multiplexing to preserve additional information such as base modifications
  • require a short preparation time
  • have limited access to laboratory equipment.

Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

 No barcodes6 barcodes12 barcodes96 barcdoes

Flow cell price

$500

$500

$500

$500

Library price

$99

$99

$99

$99

Price per sample

$599

$104

$54

$5

 

Feature

Property

Preparation time

60 minutes

Input requirement

50 ng of high molecular weight gDNA (>30 kb)

PCR Required

No

Fragmentation

Transposase-based

Read length

Random distribution, dependent on input fragment length

Read type produced

1D

Typical throughput*

••

Associated protocols

Rapid Barcoding Kit 96 (SQK-RBK110.96)

PCR tiling of SARS-CoV-2 virus with rapid barcoding (SQK-RBK110.96)

Multiplexing options

Rapid Barcoding Kit

Flow Cell Wash Kit

Pack size

3 x 96 reactions with reagents to support 12 x 24 reactions

Stability

Shipped at  2–8° C

Long-term storage  -20° C

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

The kit is optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required, some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.

The workflow is PCR-free and therefore removes PCR bias and retains base modification information which can be analysed using tools developed in the Nanopore Community.

Deconvolution of barcoded sequencing data is supported by MinKNOW and Guppy software, which classify the barcode sequence and sort reads into corresponding folders.

Further considerations:

The Rapid Barcoding Kit recommends an input of 50 ng gDNA per sample, and is optimised for samples which contain long fragments (>30 kb). Addition of less than 50 ng, or shorter fragments could compromise sequencing throughput and read length. For samples where only lower inputs are possible, the Rapid PCR Barcoding Kit is available.

Shipping and logistics:

Flow cells and kits are shipped together at 2–8°C.

Upon receipt, please place the right product in the right long-term storage location.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Workflow

The Rapid Barcoding Kit 96 generates barcoded sequencing libraries from extracted gDNA in 60 minutes using a simple protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches barcoded tags to the cleaved ends: there are 12 unique barcoded tags in the kit. Barcoded samples are pooled, purified using SPRI beads, and Rapid Sequencing Adapters are then added to the tagged ends. 

SQK-RBK110.96_gDNA_workflow_v1

Protocols
What's in the box

SQK-RBK110.96_kit_contents_v1

Name

Acronym

Cap colour

No. of tubes

Fill volume (μl)

Rapid Barcode plate

RB96

-

3 plates

2.5 μl per well

SPRI Beads

SPRI

Brown

3

960

Sequencing Buffer II

SBII

Red

1

408

Rapid Adapter F

RAP-F

Green

1

15

Loading Beads II

LBII

Pink

1

306

Elution Buffer

EB

Black

1

420

Loading Solution

LS

White cap, pink label

1

306

Flush Tether

FLT

Purple

1

360

Flush Buffer

FB

White

1 bottle

14,040

3rd party materials

Consumables

  • 1.5 ml Eppendorf DNA LoBind tubes
  • 2 ml Eppendorf DNA LoBind tubes
  • 0.2 ml thin-walled PCR tubes
  • Eppendorf twin.tec® PCR plate 96 LoBind, semi-skirted (Cat # 0030129504) with heat seals
  • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
  • Freshly-prepared 80% ethanol in nuclease-free water

Equipment

  • Ice bucket with ice
  • Microplate centrifuge, e.g. Fisherbrand™ Mini Plate Spinner Centrifuge (Fisher Scientific, #11766427)
  • Timer
  • Thermal cycler or heat block at 30°C and 80°C
  • Magnetic rack
  • Hula mixer (gentle rotator mixer)
  • P1000 pipette and tips
  • P200 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P2 pipette and tips
  • Multichannel pipette

Optional Equipment

  • Qubit fluorometer (or equivalent for QC check)
Compatibility

The Rapid Barcoding Kit 96 can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D
  • FLO-MIN111
  • FLO-PRO002
  • FLO-FLG001

Devices

  • MinION Mk1B
  • MinION Mk1C
  • GridION Mk1
  • PromethION P24/48
  • Flongle
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