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Direct RNA
Sequence RNA molecules directly and preserve base modifications
Up to 1 million reads
PCR cDNA
Optimised for throughput
Up to 12+ million reads
Direct cDNA
No PCR bias
Up to 10 million reads
Direct RNA Sequencing Kit | PCR cDNA Sequencing Kit | Direct cDNA Sequencing Kit | |
---|---|---|---|
Preparation time | 105 mins | 165 mins | 270 mins |
Input requirement | 500 ng poly-A+ RNA | 1 ng poly-A+ or 50 ng total RNA | 100 ng poly-A+ RNA |
RT Required | Optional | Yes | Yes |
PCR Required | No | Yes | No |
Read length | Equal to RNA length | Enriched for full-length cDNA | Enriched for full-length cDNA |
Typical throughput |
Less than 1 Gb in 6 hours 1-4 Gb in 48 hours |
2-3 Gb in 6 hours 8+ Gb in 48 hours |
1-2 Gb in 6 hours 4-8 Gb in 48 hours |
Typical number of reads | 1 million | 7 - 12+ million | 5 - 10 million |
Multiplexing options | In development | PCR Barcoding Kit | Native Barcoding Expansion 1-12 and Native Barcoding Expansion 13-24 |