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Direct RNA

Sequence RNA molecules directly and preserve base modifications

Up to 1 million reads

PCR cDNA

Optimised for throughput

Up to 12+ million reads

Direct cDNA

No PCR bias

Up to 10 million reads

Buy a Starter Pack    View options
 Direct RNA Sequencing KitPCR cDNA Sequencing KitDirect cDNA Sequencing Kit
Preparation time 105 mins 165 mins 270 mins
Input requirement 500 ng poly-A+ RNA 1 ng poly-A+ or 50 ng total RNA 100 ng poly-A+ RNA
RT Required Optional Yes Yes
PCR Required No Yes No
Read length Equal to RNA length Enriched for full-length cDNA Enriched for full-length cDNA
Typical throughput

One star
Less than 1 Gb in 6 hours
1-4 Gb in 48 hours

Three stars
2-3 Gb in 6 hours
8+ Gb in 48 hours

Two stars
1-2 Gb in 6 hours
4-8 Gb in 48 hours
Typical number of reads 1 million 7 - 12+ million 5 - 10 million
Multiplexing options In development PCR Barcoding Kit Native Barcoding Expansion 1-12 and
Native Barcoding Expansion 13-24

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