FAQ

Flow Cell
Online Store
Returns & Upgrades
Orders
Deliveries
Getting started with nanopore sequencing
Computer Requirements
General info
Troubleshooting
Warranty & Storage
Account & Login
Account Setup
Payment & Invoices
MinION
PromethION
MinKNOW
Software
MinION
Top Customer Services Items
Top Technical Support Items
General
Data handling
Devices
Software
Account Set-up and My Account
Deliveries and Returns
New Supplier set-up
Distributors & Dealers
Upgrades
General
General
MinIT
Frequently asked questions Products & Services
Applications

Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
View all Applications
Resources
News   Explore
Contact
You have arrived at our USA store. Select your region to see content specific to your location.
x

Your shopping basket is empty.

Flow Cell Wash Kit

EXP-WSH004

$85.00

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

  • Information

    This kit is recommended for users who:

    • want to wash flow-cells and re-run with fresh samples
    • want to run samples on demand, rather than batching samples
    • have accumulated a high number of "unavailable" pores during a previous run and wish to revert these pores to the "single pore" state
    Feature Property
    Number of washes 6
    Stability

    Shipped at  2–8° C

    Long-term storage  -20° C


    Analysis of sequence information on any Oxford Nanopore platform occurs in real-time, allowing users to run a flow cell until sufficient data has been collected to fulfil the experimental requirements, at which point the run can be stopped. The Flow Cell Wash Kit provides a highly effective means of removing a library that has been loaded onto a flow cell. Once the library has been removed, the flow cell can be re-used immediately, or stored for later usage. The washing procedure is a simple two-step process and the kit contains sufficient reagents to perform six flow cell washes.

    EXP-WSH004 is based on the use of a nuclease to digest and remove nucleic acid that has been loaded on to the flow cell. The washing procedure is very effective with as little as 0.1% of any previously loaded sample contaminating a subsequent run. However, as some contamination from the previous sample may be observed after using the Flow Cell Wash Kit, it is recommended that when running multiple samples sequentially, the samples are barcoded to allow filtering of sequences from the remnants of previously-run samples.

     

    EXP-WSH004_fig1

    Figure 1. A sample with four barcodes was sequenced. The flow cell was washed and a sample with four different barcodes was loaded. This was repeated for a third sequencing run. As show below, EXP-WSH003 and EXP-WSH004 showed effective washing of the previous samples.

    After nuclease treatment, and before storing or re-loading the flow cell, the nuclease is inactivated to prevent residual activity impacting future flow cell runs. We do not routinely observe any deterioration in read lengths in sequencing runs performed after the washing procedure.

     

     fig2

    Figure 2. The nuclease is efficiently inactivated before re-using or storing the flow cell. A sequencing library was prepared, and part of the library was loaded on to a flow cell (load #1). After a period of time, the run was stopped and a wash performed. The flow cell was re-loaded with more of the same library and the experiment re-started (load #2). The washing and re-loading steps were repeated (load #3). No difference in read length distributions was observed for the 3 library loads, suggesting very little residual nuclease activity is present after inactivation.

    Increasing flow cell output with the Flow Cell Wash Kit

    In sequencing experiments where an accumulation of pores in the “recovering” state (“unavailable” in the detailed view) is observed, the rate of data acquisition drops as fewer pores are available to accept and sequence strands. We have shown that in these circumstances, pores can be reverted to the “single pore” state by performing the washing procedure.

     

    fig3

    Figure 3. A MinION flow cell has been loaded with a sequencing library that has resulted in an accumulation of channels in the “unavailable” state, leading to a decrease in the rate of data acquisition: after 18 hours, the flow cell has <200 single pores available for sequencing from a starting point of ~1600. A washing procedure was then performed after and the number of single pores increased to ~1000, with a significant number of the channels that had been lost to the “unavailable” state reverting to the “single pore” state.

    In experiments where throughput is limited by the increase in pores in the “recovering”/“unavailable” state, we have shown that output can be at least doubled by performing several wash steps over the lifetime of a flow cell.

    fig4

    Figure 4. Throughput observed from sequencing libraries run on a PromethION flow cell. The arrows indicate the timing of each wash step: wash steps were performed at the point where the rate of data acquisition started to slow due to the accumulation of “unavailable” pores. In each case, throughput is more than doubled from the point of the first wash.

    Re-loading washed flow cells with the same library will require additional reagents contained within the Sequencing Auxiliary Vials Expansion (EXP-AUX001).

    Shipping and logistics:

    Flow cells and kits are shipped together at 2-8°C.
    Upon receipt please place the right product in the right long-term storage location.
    Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
    The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
    Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

  • Safety and legal
  • What's in the box

    EXP-WSH004_kit_contents_v2

     

    Name Acronym Cap colour Number of vials Fill volume (µl)
    Wash Mix WMX Brown 1 15
    Wash Diluent DIL Brown 2 1300
    Storage Buffer S Brown 2 1600
  • Compatibility

    The Flow Cell Wash Kit can be used together with:

    Kits

    All Oxford Nanopore sequencing kits

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D
    • FLO-MIN111
    • FLO-PRO002

    Devices

    • MinION Mk 1B
    • MinION Mk 1C
    • GridION Mk1
    • PromethION P24/P28
Information

This kit is recommended for users who:

  • want to wash flow-cells and re-run with fresh samples
  • want to run samples on demand, rather than batching samples
  • have accumulated a high number of "unavailable" pores during a previous run and wish to revert these pores to the "single pore" state
Feature Property
Number of washes 6
Stability

Shipped at  2–8° C

Long-term storage  -20° C


Analysis of sequence information on any Oxford Nanopore platform occurs in real-time, allowing users to run a flow cell until sufficient data has been collected to fulfil the experimental requirements, at which point the run can be stopped. The Flow Cell Wash Kit provides a highly effective means of removing a library that has been loaded onto a flow cell. Once the library has been removed, the flow cell can be re-used immediately, or stored for later usage. The washing procedure is a simple two-step process and the kit contains sufficient reagents to perform six flow cell washes.

EXP-WSH004 is based on the use of a nuclease to digest and remove nucleic acid that has been loaded on to the flow cell. The washing procedure is very effective with as little as 0.1% of any previously loaded sample contaminating a subsequent run. However, as some contamination from the previous sample may be observed after using the Flow Cell Wash Kit, it is recommended that when running multiple samples sequentially, the samples are barcoded to allow filtering of sequences from the remnants of previously-run samples.

 

EXP-WSH004_fig1

Figure 1. A sample with four barcodes was sequenced. The flow cell was washed and a sample with four different barcodes was loaded. This was repeated for a third sequencing run. As show below, EXP-WSH003 and EXP-WSH004 showed effective washing of the previous samples.

After nuclease treatment, and before storing or re-loading the flow cell, the nuclease is inactivated to prevent residual activity impacting future flow cell runs. We do not routinely observe any deterioration in read lengths in sequencing runs performed after the washing procedure.

 

 fig2

Figure 2. The nuclease is efficiently inactivated before re-using or storing the flow cell. A sequencing library was prepared, and part of the library was loaded on to a flow cell (load #1). After a period of time, the run was stopped and a wash performed. The flow cell was re-loaded with more of the same library and the experiment re-started (load #2). The washing and re-loading steps were repeated (load #3). No difference in read length distributions was observed for the 3 library loads, suggesting very little residual nuclease activity is present after inactivation.

Increasing flow cell output with the Flow Cell Wash Kit

In sequencing experiments where an accumulation of pores in the “recovering” state (“unavailable” in the detailed view) is observed, the rate of data acquisition drops as fewer pores are available to accept and sequence strands. We have shown that in these circumstances, pores can be reverted to the “single pore” state by performing the washing procedure.

 

fig3

Figure 3. A MinION flow cell has been loaded with a sequencing library that has resulted in an accumulation of channels in the “unavailable” state, leading to a decrease in the rate of data acquisition: after 18 hours, the flow cell has <200 single pores available for sequencing from a starting point of ~1600. A washing procedure was then performed after and the number of single pores increased to ~1000, with a significant number of the channels that had been lost to the “unavailable” state reverting to the “single pore” state.

In experiments where throughput is limited by the increase in pores in the “recovering”/“unavailable” state, we have shown that output can be at least doubled by performing several wash steps over the lifetime of a flow cell.

fig4

Figure 4. Throughput observed from sequencing libraries run on a PromethION flow cell. The arrows indicate the timing of each wash step: wash steps were performed at the point where the rate of data acquisition started to slow due to the accumulation of “unavailable” pores. In each case, throughput is more than doubled from the point of the first wash.

Re-loading washed flow cells with the same library will require additional reagents contained within the Sequencing Auxiliary Vials Expansion (EXP-AUX001).

Shipping and logistics:

Flow cells and kits are shipped together at 2-8°C.
Upon receipt please place the right product in the right long-term storage location.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

What's in the box

EXP-WSH004_kit_contents_v2

 

Name Acronym Cap colour Number of vials Fill volume (µl)
Wash Mix WMX Brown 1 15
Wash Diluent DIL Brown 2 1300
Storage Buffer S Brown 2 1600
Compatibility

The Flow Cell Wash Kit can be used together with:

Kits

All Oxford Nanopore sequencing kits

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D
  • FLO-MIN111
  • FLO-PRO002

Devices

  • MinION Mk 1B
  • MinION Mk 1C
  • GridION Mk1
  • PromethION P24/P28
Open Drift Chat to talk to our sales team