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PCR
Sequencing Kit
Control over read length
Compatible with targeted
amplicon sequencing
Rapid PCR Barcoding Kit
Simple library preparation
(15 mins hands-on time)
16S Barcoding
Genus level
bacterial identification
| PCR Sequencing Kit | Rapid PCR Barcoding Kit | 16S Barcoding Kit | |
|---|---|---|---|
| Preparation time | 60 minutes + PCR | 15 minutes + PCR | 10 minutes + PCR |
| Input recommendation | <100 ng gDNA | <10 ng gDNA | <10 ng gDNA |
| Fragmentation | Optional | Transposase based | Not required |
| Read Length | Equal to fragment length post PCR | ~2 kb | Full-length 16S gene (~1.5 kb) |
| Read type produced | 1D | 1D | 1D |
| Typical throughput |
2-3+ Gb in 6 hours 8+ Gb in 48 hours |
1-2 Gb in 6 hours 4-8 Gb in 48 hours |
1-2 Gb in 6 hours 4-8 Gb in 48 hours |
| Compatible with | Amplicon sequencing (Four primer PCR) |
||
| Multiplexing options | PCR Barcoding Kit | This kit offers barcoding for up to twelve samples | This kit offers barcoding for up to twelve samples |
