Information
The RAP T Expansion contains additional Rapid Adapter T (RAP T) and Adapter Buffer (ADB) for another six reactions. This is to enable customers to use any unused barcodes in their PCR-cDNA Barcoding Kit (SQK-PCB111.24).
To use this kit, ensure to purchase the Sequencing Auxiliary Vials (EXP-AUX002) with the Flow Cell Priming Kit (EXP-FLP002) to provide the flow cell priming reagents.
The RAP T Expansion can only be used with Kit 11 chemistry.
The RAP T Expansion properties
| Feature | Property |
|---|
| Associated kits | - PCR-cDNA Barcoding Kit (SQK-PCB111.24)
- cDNA-PCR Sequencing Kit (SQK-PCS111) |
| Pack size | 6 reactions |
| Stability | Shipped at 2–8°C
Long-term storage -20°C
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer |
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
What's in the box
The RAP T Expansion (EXP-RAP111) contains enough reagents for six reactions.
| Name | Acronym | Number of vials | Cap colour | Fill volume per vial (μl) |
|---|
| Rapid Adapter T | RAP T | 1 | Green | 10 |
| Adapter Buffer | ADB | 1 | Clear | 100 |
3rd party materials
- PCR-cDNA Barcoding Kit (SQK-PCB111.24)
- cDNA-PCR Sequencing Kit (SQK-PCS111)
To access the protocol, you will need to register for a Nanopore Community account.
Compatibility
The RAP T Expansion (EXP-RAP T) can be used together with:
Kits
- PCR-cDNA Barcoding Kit (SQK-PCB111.24)
- cDNA-PCR Sequencing Kit (SQK-PCS111)
- Flow Cell Wash Kit (EXP-WSH004)
- Sequencing Auxiliary Vials (EXP-AUX002)
- Flow Cell Priming Kit (EXP-FLP002)
Flow cells:
R9.4.1
Devices:
- MinION Mk1B
- MinION Mk1C
- GridION
- PromethION