FAQ

Flow Cell
Online Store
Returns & Upgrades
Orders
Deliveries
Getting started with nanopore sequencing
Computer Requirements
General info
Troubleshooting
Warranty & Storage
Account & Login
Account Setup
Payment & Invoices
MinION
PromethION
MinKNOW
Software
MinION
PromethION
Top Customer Services Items
Top Technical Support Items
General
Data handling
Devices
Software
Account Set-up and My Account
Deliveries and Returns
New Supplier set-up
Distributors & Dealers
Upgrades
General
General
MinIT
Frequently asked questions Products
Services
Applications
Resources
You have arrived at our USA store. Select your region to see content specific to your location.
x

Your shopping basket is empty.

16S Barcoding Kit

SQK-RAB204

$650.00

Genus level bacterial identification with barcoding for up to 12 samples.

Includes a Flow Cell Priming Kit

gDNA 10 ng ~10 mins + PCR PCR Bacterial ID
  • Information

    This kit is recommended for users who

    • wish to multiplex samples to reduce price per sample
    • want to do 16S sequencing
    • are interested in genus level bacterial identification

    Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow-cell: it allows a user to pool multiple samples and sequence them together making more efficient use of the flow-cell.

     Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

     No barcodes6 barcodes12 barcodes

    Flow cell price

    $500

    $500

    $500

    Library price

    $99

    $99

    $99

    Barcode price

    -

    $25

    $50

    Price per sample

    $599

    $104

    $54

     

    The 16S Barcoding Kit offers a method of amplifying and barcoding the ~1500 bp 16S rRNA gene from multiple samples and sequencing them together. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical.

    The 16S Barcoding Kit features:

    Feature Property
    Preparation time 10 minutes + PCR
    Input requirement <10 ng gDNA
    PCR Required Yes
    Fragmentation Not required
    Read length Full-length 16S gene (~1.5 kb)
    Read type produced 1D
    Typical throughput* ••
    Associated protocols

    16S Barcoding Kit

    Flow Cell Wash Kit

    Pack size 6 reactions
    Stability

    Shipped at  2-8° C

    Long-term storage  -20° C

     

    The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain barcodes and 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 12x barcoded primer pairs.

    The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

    Further considerations:

    Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

  • Workflow

    The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain barcodes and 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 12x barcoded primer pairs.

    The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Albacore and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

  • Protocols
  • Safety and legal
  • What's in the box

    The 16S Barcoding Kit contains 12 unique barcodes and sufficient reagents to generate six sequencing libraries.

     

     

    The Flow Cell Priming Kit Expansion Pack is also supplied.

    EXP-FLP002

  • 3rd party materials

    Consumables

    • Agencourt AMPure XP beads
    • 1.5 ml Eppendorf DNA LoBind tubes
    • 0.2 ml thin-walled PCR tubes
    • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
    • Freshly prepared 70% ethanol in nuclease-free water
    • LongAmp Taq 2X Master Mix (e.g. NEB M0287)
    • 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

    Equipment

    • Microfuge
    • Timer
    • Thermal cycler
    • P1000 pipette and tips
    • P100 pipette and tips
    • P20 pipette and tips
    • P10 pipette and tips
    • P2 pipette and tips

    Optional Equipment

    • Agilent Bioanalyzer (or equivalent)
    • Qubit fluorometer (or equivalent for QC check)
    • Eppendorf 5424 centrifuge (or equivalent)
  • Compatibility

    The PCR Sequencing Kit can be used together with:

    Kits

    • RAP Top-up Kit (EXP-RAP001)
    • Flow Cell Wash Kit (EXP-WSH003)

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D

    Devices

    • MinION Mk 1B
    • MinION Mk 1C
    • GridION Mk1
Information

This kit is recommended for users who

  • wish to multiplex samples to reduce price per sample
  • want to do 16S sequencing
  • are interested in genus level bacterial identification

Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow-cell: it allows a user to pool multiple samples and sequence them together making more efficient use of the flow-cell.

 Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples:

 No barcodes6 barcodes12 barcodes

Flow cell price

$500

$500

$500

Library price

$99

$99

$99

Barcode price

-

$25

$50

Price per sample

$599

$104

$54

 

The 16S Barcoding Kit offers a method of amplifying and barcoding the ~1500 bp 16S rRNA gene from multiple samples and sequencing them together. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical.

The 16S Barcoding Kit features:

Feature Property
Preparation time 10 minutes + PCR
Input requirement <10 ng gDNA
PCR Required Yes
Fragmentation Not required
Read length Full-length 16S gene (~1.5 kb)
Read type produced 1D
Typical throughput* ••
Associated protocols

16S Barcoding Kit

Flow Cell Wash Kit

Pack size 6 reactions
Stability

Shipped at  2-8° C

Long-term storage  -20° C

 

The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain barcodes and 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 12x barcoded primer pairs.

The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

Further considerations:

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Workflow

The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain barcodes and 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters. The kit contains 12x barcoded primer pairs.

The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Albacore and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

What's in the box

The 16S Barcoding Kit contains 12 unique barcodes and sufficient reagents to generate six sequencing libraries.

 

 

The Flow Cell Priming Kit Expansion Pack is also supplied.

EXP-FLP002

3rd party materials

Consumables

  • Agencourt AMPure XP beads
  • 1.5 ml Eppendorf DNA LoBind tubes
  • 0.2 ml thin-walled PCR tubes
  • Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
  • Freshly prepared 70% ethanol in nuclease-free water
  • LongAmp Taq 2X Master Mix (e.g. NEB M0287)
  • 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

Equipment

  • Microfuge
  • Timer
  • Thermal cycler
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips
  • P2 pipette and tips

Optional Equipment

  • Agilent Bioanalyzer (or equivalent)
  • Qubit fluorometer (or equivalent for QC check)
  • Eppendorf 5424 centrifuge (or equivalent)
Compatibility

The PCR Sequencing Kit can be used together with:

Kits

  • RAP Top-up Kit (EXP-RAP001)
  • Flow Cell Wash Kit (EXP-WSH003)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • MinION Mk 1B
  • MinION Mk 1C
  • GridION Mk1
Open Drift Chat to talk to our sales team