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This kit is recommended for users who:
- require a short preparation time
- have limited access to laboratory equipment.
The Rapid Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
The Rapid Sequencing Kit features:
| Feature | Property |
| Preparation time | 10 minutes |
| Input requirement | 400 ng of high molecular weight gDNA (>30 kb) |
| PCR Required | No |
| Fragmentation | Transposase-based |
| Read length | Random distribution, dependent on input fragment length |
| Read type produced | 1D |
| Typical throughput* | ••• |
| Associated protocols |
Rapid Sequencing Kit Rapid whole genome amplification |
| Multiplexing options |
Rapid Barcoding Kit Flow Cell Wash Kit |
| Pack size | 6 reactions |
| Stability |
Shipped at 2–8° C Long-term storage -20° C |
The kit is optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required (e.g. minimal pipetting steps and no clean-ups) some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.
Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.
Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.
The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.
Further considerations:
The Rapid Sequencing Kit recommends an input of 400 ng gDNA, and is optimised for samples which contain long fragments (>30 kb). Addition of less than 400 ng, or the presence of shorter fragments could compromise sequencing throughput and read length. For samples where only lower inputs are possible, the Low Input by PCR Sequencing Kit and Rapid Low Input by PCR Sequencing Kit are available.
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.
