Information
This kit is recommended for users who:
- Require a short preparation time
- Would like to do automated library preparation on the VolTRAX V2b device
The VolTRAX Sequencing Kit (VSK-VSK004) is an upgrade to the VSK-VSK003 and generates sequencing libraries from extracted gDNA in 5-10 minutes hands-on time plus 45 minutes automation. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Sequencing Adapters are then added to the tagged ends, and the library is cleaned up using AMPure XP Beads.
The kit is optimised for simplicity, reducing hands-on time, and increased throughput. Due to the simple nature of the workflow and the precision of fluid handling on the VolTRAX V2b platform, the consistency in performance of libraries generated with this kit is greater than with other kits, and is less affected by user familiarity with library preparation.
VolTRAX Sequencing Kit features:
| Feature | Property |
|---|
| Preparation time | Hands on time: 5-10 mins
Automation: 45 mins |
| Input requirement | 475 ng extracted DNA |
| PCR required | No |
| Fragmentation | Transposase-based |
| Read length | Random distribution, dependent on input fragment length |
| Read type produced | 1D |
| Associated protocols | VolTRAX V2b protocol |
| Pack size | 3 reactions |
| Stability | Shipped at 2-8°C
Long-term storage -20°C, unopened, for 3 months |
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Protocols
VolTRAX protocol
To access the protocol, you will need to register for a Nanopore Community account.
What's in the box
The VolTRAX Sequencing Kit contains sufficient reagents to generate three sequencing libraries.
| Name | Acronym | Cap colour | Number of vials | Fill volume (µl) |
|---|
| VX Fragmentation Mix | VFA | Amber | 1 | 25 |
| EDTA | EDTA | Clear | 1 | 20 |
| VX AMPure XP Beads | VXP | Pink | 1 | 30 |
| VX Long Fragment Buffer | VLF | Orange | 1 | 40 |
| VX Rapid Adapter T | VRA T | Green | 1 | 25 |
| VX Elution Buffer | VEB | Black | 1 | 65 |
| VX Running Buffer | VRB | Red | 1 | 250 |
| Flush Buffer | FB | Blue | 3 | 1,170 |
| Flush Tether | FLT | White cap, purple label | 1 | 200 |
Compatibility
The VolTRAX Sequencing Kit can be used together with:
Kits
- Flow Cell Wash Kit (EXP-WSH004)
Flow cells
Devices
The library prepared on the VolTRAX can be sequenced on:
- MinION Mk1B
- MinION Mk1C
- GridION
Cartridge