COVID Midi V14: 2304 samples

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples using our V14 chemistry.

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  • Product Warranty may initially be limited to 1 month as it is so new
  • Product routinely iterates as new features and capabilities are added
  • Estimated lead time in store
  • Product subject to availability


A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples:

  • Midnight Expansion Kit with RT-PCR reagents and primers to amplify SARS-CoV-2 from input RNA
  • Rapid Barcoding Kits 96 V14 to barcode and prepare the amplified material for sequencing
  • MinION/GridION Flow Cells to perform sequencing on MinION or GridION devices
  • Sequence for as little as $10.51 per sample

How it works

The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long). By amplifying longer amplicons, Oxford Nanopores rapid chemistry can be deployed enabling users to rapidly attach a barcode, pool samples and adapt them for sequencing. This method is simple and quick to deploy. It allows rapid turn-around-times that can assist with monitoring of novel variants of concern.

The protocol delivers robust full genome coverage across a wide range of Ct values.

Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow.

image2021-9-8 17-48-8

The Midnight bundle includes everything you need to sequence SARS-CoV-2:

FeatureMidnight bundle
Preparation time5 h 30 min including RT-PCR
Hands-on time1 h 15 min
Input amount8 μl extracted RNA
Multiplexingrun in sets of 24* - 96 samples per flow cell
Pack size2,304 samples
StorageShipped at 2-8°C

Flow cells: long-term storage 2-8°C

Kits: long-term storage -20°C
CompatibilityMinION Mk1B
GridION Mk1
Please contact us for PromethION
3rd party reagents requiredEthanol, nuclease-free water

*users wishing to run in multiples of 24 will need to buy 12 x Flow Cell Wash Kits (EXP-WSH004). *users wishing to run in multiples of 48 will need to buy 4 x Flow Cell Wash Kits (EXP-WSH004).

Shipping and logistics:

Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.


The sample preparation workflow is simple, flexible and rapid.

image2021-9-8 17-17-20

What's in the box

This bundle contains:

  • 4 x Midnight Expansion Kit (EXP-MRT001)
  • 8 x Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
  • 24 x MinION/GridION Flow Cells (FLO-MIN114)


The Midnight Bundle can be used together with:


  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MIN114


  • MinION Mk1B
  • MinION Mk1C
  • GridION Mk1