Additional Rapid Adapter T (RAP T) to maximise the use of the PCR-cDNA Barcoding Kit (SQK-PCB111.24).

Note: This is only compatible with Kit 11 chemistry.

Product lead time: 3 weeks

Additional flow cell priming reagents for our V14 kits.

This is an Early Access product

Note: This is only compatible with V14 chemistry.

Product lead time: 2 weeks

Additional Rapid Adapter and Adapter Buffer to enable customers to maximise the use of Rapid Barcoding Kits 24 and 96 V14 (SQK-RBK114.24 and SQK-RBK114.96).

This is an Early Access product

Note: This is only compatible with V14 chemistry

Additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96).

This is an Early Access product

Note: This is only compatible with V14 chemistry

RNA Control Sample to support control runs for cDNA kits.

This is an Early Access product

Additional flow cell priming reagents for our V14 kits.

This is an Early Access product

Note: This is only compatible with V14 chemistry

The Sequencing Auxiliary Vials contain Sequencing Buffer (SB), Elution Buffer (EB), Library Solution (LIS) and Library Beads (LIB) with V14 flow cell priming reagents: Flow Cell Flush (FCF) and Flow Cell Tether (FCT).

This is an Early Access product

Note: This is only compatible with V14 chemistry.

An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: ~200 minutes + overnight elution
• Read length: N50 >50 kb
• Input amount: 6 million cells

Rapid and simple method of preparing up to 24 barcoded samples for sequencing from 1-5 ng of gDNA using PCR.

This is an Early Access product

This uses our latest Kit 14 chemistry

Product lead time: 2 weeks

• Preparation time: 15 minutes + PCR
• Read length: ~2 kb
• Input amount: 1-5 ng gDNA per sample

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

Simple and rapid library preparation, with barcoding for up to 24 gDNA samples.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 100 ng dsDNA

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This is an Early Access product

Product lead time: 1 week

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = to fragment length
• Input amount: 400 ng per sample of gDNA, 200 fmol per sample (130 ng for 1 kb amplicons)

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This is an Early Access product

Product lead time: 1 week

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400-1000 ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.

This uses our latest Kit 14 chemistry

• Preparation time: 120 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of  amplicons or cDNA

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR,100-200 fmol of  amplicons or cDNA

An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 24 samples.

This uses our Kit 11 chemistry

This is an Early Access product

• Preparation time: 210 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA per sample

A sequencing kit optimised for output and PCR-free sequencing of 2-3 samples for Whole Genome Sequencing (WGS).

This is an Early Access product

This uses our Kit 11 chemistry

• Preparation time: 135 minutes
• Read length: = Fragment length
• Input amount: 1000 ng dsDNA per sample

The Flow Cell Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells on a higher scale.

This is compatible with all DNA sequencing kits.

Identification and quantification of full-length transcripts with highest output.

This uses our Kit 11 chemistry

• Preparation time: 210 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

Product lead time: 3 weeks

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

Product lead time: 3 weeks

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

Product lead time: 3 weeks

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

Product lead time: 1 week

This is compatible with Kit 10 and Kit 14 chemistry

• Preparation time: 80 minutes + PCR

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

This is compatible with all DNA sequencing kits.

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples. We recommend using our most recent version: Rapid Barcoding Kit 96 V14 (SQK-RBK114.96).

This uses Kit 10 chemistry.

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

Prepare RNA for direct RNA nanopore sequencing.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 115 minutes
• Read length: = RNA length
• Input amount: 500 ng total RNA, 50 ng poly(A)+ RNA

Genus-level bacterial identification with barcoding for up to 24 samples.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes + PCR
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng dsDNA per sample

Targeted sequencing of genomic regions using CRISPR-Cas9.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 110 minutes
• Read length: = fragment length
• Input amount: 1–10 µg of dsDNA

Lambda DNA (LMD). Pack of six vials

This is compatible with all DNA sequencing kits

PCR Primers (PRM) and PCR Adapters (PCA)

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries.

• Preparation time: 150 minutes
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries.

• Preparation time: 150 minutes
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

Additional Long Fragment Buffer (LFB).

Note: This is compatible with all kit chemistries.

Product lead time: 2 weeks

Additional Short Fragment Buffer (SFB)

Note: This is compatible with all kit chemistries.