Information
This kit is recommended for users who:
- Want to wash flow cells and rerun with fresh samples.
- Wish to run samples on demand, rather than batching samples.
- Have accumulated a high number of "unavailable" pores during a previous run and wish to revert these pores to the "single pore" state.
The Wash Kit features:
| Feature | Property | | ---------- | ---------- | | Associated protocols | Please refer to the Protocols tab. | | Associated kits | All sequencing kits | | Pack size | 48 reactions |
| Storage | -20°C to -30°C |
| Stability | This kit is stable during shipping for 7 days at temperatures up to 25ºC. Upon receipt, please store the kit at -20°C to -30°C.
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer. |
Nanopore technology allows for real-time assessment of sequencing experiments with the visualisation of run statistics on the instrument via the MinKNOW user interface. This allows users to run a flow cell until sufficient data has been collected to fulfil the experimental requirements, at which point the run can be stopped. The Flow Cell Wash Kit provides a highly effective means of removing a library that has been loaded onto a flow cell. Once the library has been removed, the flow cell can be re-used immediately, or stored for later usage. The washing procedure is a simple two-step process and the kit contains sufficient reagents to perform 48 flow cell washes.
The Flow Cell Wash Kit XL (EXP-WSH004-XL) is based on the use of a nuclease to digest and remove nucleic acid that has been loaded on to the flow cell. The washing procedure is very effective, with as little as 0.1% of any previously loaded sample contaminating a subsequent run. However, as some contamination from the previous sample may be observed after using the Flow Cell Wash Kit, it is recommended that when running multiple samples sequentially, the samples are barcoded to allow filtering of sequences from the remnants of previously run samples.
Re-loading washed flow cells with the same library will require additional reagents contained within the Sequencing Auxiliary Vials V14 (EXP-AUX003 or EXP-AUX003-XL).
Please refer to the Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) know-how for more information on this kit.
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Compatibility
The Flow Cell Wash Kit can be used together with:
Kits
All Oxford Nanopore DNA sequencing kits
Flow cells
Devices
- MinION
- GridION
- PromethION
