Information
The Native Barcoding Auxiliary V14 (EXP-NBA114) expansion contains additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96), and the Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL), for another 12 reactions. This is to enable customers to use any unused barcodes in their Native Barcoding Kits.
To use this kit, ensure to purchase the Sequencing Auxiliary Vials V14 (EXP-AUX003) to provide the flow cell priming reagents.
The Native Barcoding Auxiliary Kit V14 (EXP-NBA114) can only be used with Kit 14 chemistry. This kit is NOT compatible with our previous kit chemistries.
The Native Barcoding Auxiliary Kit V14 features:
| Feature | Property |
|---|
| Associated kits | • Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Native Barcoding Kit 96 V14 (SQK-NBD114.96) |
| Kit chemistry | Kit 14 (V14) |
| Pack size | 12 reactions |
| Stability | Shipped at 2–8°C
Long-term storage -20°C
The warranty for this Early Access product is 3 months from receipt by the customer |
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
What's in the box
The Native Barcoding Auxiliary Kit V14 (EXP-NBA114) contains enough reagents for another 12 reactions.
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (µl) |
|---|
| Native Adapter | NA | Green | 2 | 40 |
| AMPure XP Beads | AXP | Amber | 1 | 400 |
| Long Fragment Buffer | LFB | Orange | 2 | 1,800 |
| Short Fragment Buffer | SFB | Clear | 2 | 1,800 |
Note: This Product Contains AMPure XP Reagent Manufactured by Beckman Coulter, Inc. and can be stored at -20°C with the kit without detriment to reagent stability.
Compatibility
The Native Barcoding Auxiliary Kit V14 can be used together with:
Kits:
- Native Barcoding Kit 24 V14 (SQK-NBD114.24)
- Native Barcoding Kit 96 V14 (SQK-NBD114.96)
- Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL)
- Sequencing Auxiliary Vials V14 (EXP-AUX003)
- Flow Cell Wash Kit (EXP-WSH004)
- Flow Cell Priming Kit (EXP-FLP004)
- Flow Cell Priming Kit XL (EXP-FLP004-XL)
Flow cells:
R10.4.1
- FLO-MIN114
- FLO-PRO114M
- FLO-FLG114
Devices:
- Flongle
- MinION Mk1B
- MinION Mk1C
- GridION
- PromethION