Information
The Native Barcoding Auxiliary V14 (EXP-NBA114) expansion contains additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96), and the Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL), for another 12 reactions. This is to enable customers to use any unused barcodes in their Native Barcoding Kits.
To use this kit, ensure to purchase the Sequencing Auxiliary Vials V14 (EXP-AUX003) to provide the flow cell priming reagents.
The Native Barcoding Auxiliary Kit V14 (EXP-NBA114) can only be used with Kit 14 chemistry. This kit is NOT compatible with our previous kit chemistries.
The Native Barcoding Auxiliary Kit V14 features:
| Feature | Property |
|---|
| Associated kits | • Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Native Barcoding Kit 96 V14 (SQK-NBD114.96) |
| Kit chemistry | Kit 14 (V14) |
| Pack size | 12 reactions for standard methods
Application specific methods may require additional reagents. |
| Storage | −20°C to −30°C |
| Stability | Upon receipt, immediately store kits between −20°C and −30°C until you are ready to use them.
Kits are stable for up to 10 freeze/thaw cycles.
The warranty for this product is 3 months from receipt by the customer. |
Shipping and logistics:
Expansion kits are sensitive to temperature. To maintain their temperature and ensure optimal results during use, consumables are shipped with cool packs. It is not unusual for the cool packs to be warm when you receive them. For optimal results, you should immediately store your new kits at their correct temperatures.
We recommend that expansion kits should be stored between −20°C and −30°C until you are ready to use them. Kits are stable for up to 10 freeze/thaw cycles.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
What's in the box
The Native Barcoding Auxiliary Kit V14 (EXP-NBA114) contains enough reagents for another 12 reactions.
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (µl) |
|---|
| Native Adapter | NA | Green | 2 | 40 |
| AMPure XP Beads | AXP | Amber | 1 | 400 |
| Long Fragment Buffer | LFB | Orange | 2 | 1,800 |
| Short Fragment Buffer | SFB | Clear | 2 | 1,800 |
Note: This product contains AMPure XP reagent manufactured by Beckman Coulter Inc. and can be stored between −20°C and −30°C with the kit, without detriment to reagent stability.
Compatibility
The Native Barcoding Auxiliary Kit V14 can be used together with:
Kits:
- Native Barcoding Kit 24 V14 (SQK-NBD114.24)
- Native Barcoding Kit 96 V14 (SQK-NBD114.96)
- Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL)
- Sequencing Auxiliary Vials V14 (EXP-AUX003)
- Flow Cell Wash Kit (EXP-WSH004)
- Flow Cell Priming Kit (EXP-FLP004)
- Flow Cell Priming Kit XL (EXP-FLP004-XL)
Flow cells:
- FLO-MIN114
- FLO-PRO114M
- FLO-FLG114
Devices:
- Flongle
- MinION
- GridION
- PromethION
