Information
This kit is highly recommended for users who:
- Would like to remove PCR bias
- Are exploring attributes of native RNA such as modified bases
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd party materials" tab for more detail.
The Q-Line Direct RNA Sequencing Kit features:
| Feature | Property |
|---|
| Preparation time | ~140 minutes |
| Input requirement | 300 ng poly(A) tailed RNA or 1 μg total RNA |
| PCR required | No |
| Fragmentation | No |
| Read length | RNA length |
| Pack size | 6 reactions |
| Storage | −30°C to −20°C |
| Stability | Store at –30°C to –20°C until the labelled expiration date.
Q reagents are supplied with a minimum of 3 months shelf life remaining at the time of delivery.
Expiration dates are displayed in DD-MMM-YYYY format. |
Shipping and logistics:
Bulk order options are available. All orders can be split for delivery to best suit your needs.
Sequencing kits are sensitive to temperature. To maintain their temperature and ensure optimal results during use, consumables are shipped with cool packs. It is not unusual for the cool packs to be warm when you receive them. For optimal results, you should immediately store your new kits at their correct temperatures.
We recommend that sequencing kits should be stored between −30°C and −20°C until you are ready to use them.
Workflow
The Q-Line Direct RNA Sequencing Kit (Q-SQK-RNA004) is used to prepare either poly(A)-tailed RNA or total RNA for sequencing by performing reverse transcription to synthesise a secondary complementary cDNA strand for stability to improve sequencing output. Sequencing adapters are then attached to the RNA-cDNA hybrid for sequencing.
What's in the box
The Q-Line Direct RNA Sequencing Kit contains enough reagents for six reactions.
| Name | Abbreviation | Cap colour | Quantity | Minimum fill volume per container (μl) |
|---|
| RT Adapter | RTA | Blue | 1 | 6 |
| RNA Ligation Adapter | RLA | Green | 1 | 36 |
| Wash Buffer | WSB | Orange | 2 | 900 |
| RNA Elution Buffer | REB | Amber | 1 | 78 |
| Library Solution | LIS | White cap, pink label | 1 | 153 |
| Sequencing Buffer | SB | Red | 1 | 225 |
| RNA Flush Tether | RFT | Violet | 1 | 180 |
| Flow Cell Flush | FCF | N/A | 1 | 7,020 |
Compatibility
The Q-Line Direct RNA Sequencing Kit can be used together with:
Flow cells
- Q-Line MinION Flow Cell RNA (Q-FLO-MIN004R)
Devices
- GridION Q sequencing device (Q-GRD-MK1)
