Information
This kit is recommended for users who:
- Want to size select to progressively deplete short fragments (<25 kb) in their sample
- Want to enrich their libraries for long fragments without using any external third-party reagents
The Short Fragment Eliminator (EXP-SFE001) is a new expansion pack that is recommended for users who want to size select high molecular weight (HMW) gDNA to deplete short fragments (<25 kb) using size selective precipitation. The kit contains Short Fragment Eliminator (SFE) buffer which progressively removes fragments up to 25 kb, with fragments under 10 kb being almost completely removed in a quick and simple workflow.
Read lengths observed when HMW gDNA was prepared using the Ligation Sequencing Kit with and without size selection (EXP-SFE001 treatment).
Note: This kit is designed to remove short DNA fragments and requires samples to be predominantly made up of HMW DNA (>48 kb). If there are large amounts of short fragments in your sample, this will result in a low yield at the end of the prep and a low sequencing output.
The Short Fragment Eliminator kit properties
| Feature | Property |
|---|
| Associated kits | All DNA sequencing kits |
| Pack size | 30 reactions |
| Kit chemsitry | Compatible with all DNA kits |
| Stability | Shipped at 2–25°C
Long-term storage 18-25°C
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer |
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Workflow
DNA is extracted and quantified for length, quality and purity before precipitating the sample with the SFE buffer and pelleting the DNA. The DNA is carefully washed with ethanol and resuspended in nuclease-free water for immediate library prep or TE buffer for long-term storage.
Protocols
Size selection of HMW DNA using Short Fragment Eliminator (EXP-SFE001)
To access the protocols, you will need to register for a Nanopore Community account.
Compatibility
The Short Fragment Eliminator Expansion can be used together with:
Kits
Flow cells
- FLO-MIN106D
- FLO-MIN114
- FLO-PRO002
- FLO-PRO114M
- FLO-FLG001
- FLO-FLG114
Devices
- MinION Mk1B
- MinION Mk1C
- GridION
- PromethION