FAQ

Flow Cell
Online Store
Returns & Upgrades
Orders
Deliveries
Getting started with nanopore sequencing
Computer Requirements
General info
Troubleshooting
Warranty & Storage
Account & Login
Account Setup
Payment & Invoices
MinION
PromethION
MinKNOW
Software
MinION
PromethION
Top Customer Services Items
Top Technical Support Items
General
Data handling
Devices
Software
Account Set-up and My Account
Deliveries and Returns
New Supplier set-up
Distributors & Dealers
Upgrades
General
General
MinIT
Frequently asked questions Products
Services
Applications
Resources
You have arrived at our USA store. Select your region to see content specific to your location.
x

Your shopping basket is empty.

PCR Barcoding Expansion 1-12

EXP-PBC001

$288.00

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

gDNA / amplicon / cDNA ~150 mins + PCR PCR
  • Information

    The PCR Barcoding Expansion 1-12 is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION. The kit includes adapters and Oxford Nanopore forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes and enough of the required adapters to make 6 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimise the opportunity for cross-talk. There are currently three protocols for this kit, which are provided as examples of how the PCR Barcoding Expansion 1-12 for genomic, amplicon, and cDNA libraries work. Please note that if you are barcoding cDNA or amplicons, these have to be pre-made in advance. These protocols are open for adaptation and optimisation for specific experimental requirements. This kit is used with the Ligation Sequencing Kit for library preparation. Store at -20 °C.

    Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

  • Workflow

  • Protocols

    Example experiment using the PCR Barcoding Expansion 1-12 with the Ligation Sequencing Kit (EXP-PBC001 & SQK-LSK109) - for illustration purposes only

  • Safety and legal
  • What's in the box

  • 3rd party materials

    In addition to the equipment and consumables used with the Ligation Sequencing Kit:

    • LongAmp Taq 2X Master Mix (e.g. NEB cat # M0287)
  • Compatibility

    The PCR Barcoding Expansion 1-96 can be used together with:

    Kits

    • Ligation Sequencing Kit (SQK-LSK109)
    • Flow Cell Wash Kit (EXP-WSH003)

    Flow cells

    • FLO-MINSP6
    • FLO-MIN106D

    Devices

    • MinION Mk 1B
    • MinION Mk 1C
    • GridION Mk1
Information

The PCR Barcoding Expansion 1-12 is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION. The kit includes adapters and Oxford Nanopore forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes and enough of the required adapters to make 6 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimise the opportunity for cross-talk. There are currently three protocols for this kit, which are provided as examples of how the PCR Barcoding Expansion 1-12 for genomic, amplicon, and cDNA libraries work. Please note that if you are barcoding cDNA or amplicons, these have to be pre-made in advance. These protocols are open for adaptation and optimisation for specific experimental requirements. This kit is used with the Ligation Sequencing Kit for library preparation. Store at -20 °C.

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Workflow

Protocols

Example experiment using the PCR Barcoding Expansion 1-12 with the Ligation Sequencing Kit (EXP-PBC001 & SQK-LSK109) - for illustration purposes only

What's in the box

3rd party materials

In addition to the equipment and consumables used with the Ligation Sequencing Kit:

  • LongAmp Taq 2X Master Mix (e.g. NEB cat # M0287)
Compatibility

The PCR Barcoding Expansion 1-96 can be used together with:

Kits

  • Ligation Sequencing Kit (SQK-LSK109)
  • Flow Cell Wash Kit (EXP-WSH003)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • MinION Mk 1B
  • MinION Mk 1C
  • GridION Mk1
Open Drift Chat to talk to our sales team