Vials of Adapter Mix II

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109.

• Preparation time: 60 minutes
• Throughput: ★★★
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

A simple and rapid library preparation method for genomic DNA on the VolTRAX, with barcoding for up to 10 gDNA samples.

• Preparation time: 10 minutes
• Throughput: 10 – 20 Gb in 48 hours
• Read length: Random distribution, dependent on input fragment length
• Input amount: 55 ng dsDNA

The VolTRAX V2 Cartridge Pack contains three disposable cartridges that interface with the VolTRAX V2 device.

• Preparation time: 5 minutes
• Throughput: 10 - 20 GB in 48 hours
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

A ligation-based sequencing kit for multiplexing samples.
For singleplex samples, please use our new version: SQK-LSK110. All COVID-related projects will be supported indefinitely. For other projects, we will discontinue the kit on Sept 10, 2021.

• Preparation time: 60
• Throughput: ★★★
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple and rapid library preparation method for genomic DNA.

• Preparation time: 10
• Throughput: ★★☆
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

• Preparation time: PCR + 60
• Throughput: ★★★
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

• Preparation time: PCR + 15
• Throughput: ★★☆
• Read length: ~2 kb
• Input amount: 1-5 ng gDNA

Ultra-Long DNA Sequencing Kit

• Preparation time: 610 minutes
• Throughput: ★★★
• Read length: N50 >50 kb

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR.

• Preparation time: PCR + 60
• Throughput: ★★★
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples

• Preparation time: 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples.

• Preparation time: 10
• Throughput: ★★☆
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

Identification and quantification of full-length transcripts without PCR bias.

• Preparation time: 270
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
• Read length: Enriched for full-length cDNA
• Input amount: 100 ng poly-A+ RNA

Identification and quantification of full-length transcripts with highest throughput

• Preparation time: 165
• Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
• Read length: Enriched for full-length cDNA
• Input amount: 1 ng poly-A+ or 50 ng total RNA

Prepare RNA for direct RNA nanopore sequencing

• Preparation time: 115
• Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION
• Read length: = RNA length
• Input amount: 500 ng RNA (poly-A+)

Cold chain-free library preparation for ultimate portability.

• Preparation time: 10
• Throughput: ★★☆
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

Genus-level bacterial identification with barcoding for up to 24 samples.

• Preparation time: PCR + 10
• Throughput: ★★☆
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

• Preparation time: 130
• Throughput: ★★★
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

• Preparation time: 165
• Throughput: ★★★
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA

Targeted sequencing of genomic regions using CRISPR-Cas9

• Preparation time: 110
• Throughput: ★☆☆
• Read length: = fragment length
• Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time.

• Preparation time: PCR + 10
• Throughput: ★★☆
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

Lambda DNA (LMD). Pack of six vials

PCR Primers (PRM) and PCR Adapters (PCA)

Rapid Sequencing Adapters (RAP)

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode.

• Preparation time: 150'
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes, sufficient for 6 reactions.

• Preparation time: 150'
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

• Preparation time: 80'
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
• Read length: = to fragement length
• Input amount: 1000 ng dsDNA

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

• Preparation time: 80'
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
• Read length: = to fragement length
• Input amount: 1000 ng dsDNA

Short Fragment Buffer (SFB)

Flow Cell Priming Kit XL

The Sequencing Auxiliary Vials contain Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS) and Loading Beads II (LBII).

The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or PromethION flow cells.

Flow Cell Priming Kit

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

• Preparation time: PCR + 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

A simple and rapid library preparation method for genomic DNA on the VolTRAX

• Preparation time: 10 minutes
• Throughput: 10 – 20 Gb in 48 hours
• Read length: Random distribution, dependent on input fragment length
• Input amount: 475 ng dsDNA