A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This is an Early Access product

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

Additional library preparation reagents for the Native Barcoding Kit 24 and 96 (SQK-NBD112.24 and SQK-NBD112.96).

This is an Early Access product

This product has a 2 week lead time

• Read length: = to fragment length
• Input amount: 1000 ng dsDNA

The Flow Cell Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells on a higher scale.

This product has a 6 week lead time

Identification and quantification of full-length transcripts with highest output.

This product has a 6 week lead time

• Preparation time: 160 + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly-A+ RNA, or 200 ng total RNA

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 12 samples.

This is an Early Access product

This product has a 6 week lead time

• Preparation time: 160 + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly-A+ RNA, or 200 ng total RNA

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long reads.

This is an Early Access product

This product has a 2 week lead time

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400 ng high moleculare weight dsDNA per sample, 200 fmol (130 ng for 1 kb amplicons) per sample

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long reads.

This is an Early Access product

This product has a 2 week lead time

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA for <4 barcodes, 400 ng high moleculare weight dsDNA for >4 barcodes

A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long reads. Multiple samples can be processed simultaneously.

This is an Early Access product

This product has a 6 week lead time

• Preparation time: 120 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

• Preparation time: 80 minutes + PCR
• Read length: = to fragment length post-PCR

Vials of Adapter Mix II

This product has a 2 week lead time

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples, we recommend using the sequencing kits: Native Barcoding Kit 24 (SQK-NBD112.24) or the Native Barcoding Kit 96 (SQK-NBD112.96) which use our Kit 12 leading chemistry.

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

A ligation-based sequencing kit for multiplexing samples.
For singleplex samples, please use our new version: SQK-LSK112. All COVID-related projects will be supported indefinitely. For other projects, we will discontinue the kit 3 months after the introduction of the Native Barcoding Kit 24 (SQK-NBD112.24) and the Native Barcoding Kit 96 (SQK-NBD112.96). Includes a Flow Cell Priming Kit

• Preparation time: 60
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple and rapid library preparation method for genomic DNA.

Includes a Flow Cell Priming Kit

• Preparation time: 10
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 60
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 15
• Read length: ~2 kb
• Input amount: 1-5 ng gDNA

Ultra-Long DNA Sequencing Kit

• Preparation time: 610 minutes
• Read length: N50 >50 kb

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 60
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples

This product has a one-week lead time

• Preparation time: 60
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng HMW gDNA

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples.

Includes a Flow Cell Priming Kit

• Preparation time: 10
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

Identification and quantification of full-length transcripts without PCR bias.

Includes a Flow Cell Priming Kit

• Preparation time: 275
• Read length: Enriched for full-length cDNA
• Input amount: 100 ng poly-A+ RNA

Identification and quantification of full-length transcripts with highest throughput

Includes a Flow Cell Priming Kit

• Preparation time: 165
• Read length: Enriched for full-length cDNA
• Input amount: 1 ng poly-A+ or 50 ng total RNA

Prepare RNA for direct RNA nanopore sequencing

Includes a Flow Cell Priming Kit

• Preparation time: 115
• Read length: = RNA length
• Input amount: 500 ng RNA (poly-A+)

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

• Preparation time: 10
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 10
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

• Preparation time: 130
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

• Preparation time: 165
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA

Targeted sequencing of genomic regions using CRISPR-Cas9

Includes a Flow Cell Priming Kit

• Preparation time: 110
• Read length: = fragment length
• Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 10
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

Lambda DNA (LMD). Pack of six vials

PCR Primers (PRM) and PCR Adapters (PCA)

Rapid Sequencing Adapters (RAP)

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode.

• Preparation time: 150'
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes, sufficient for 6 reactions.

• Preparation time: 150'
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

• Preparation time: 80
• Read length: = to fragment length
• Input amount: 1000 ng dsDNA

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

• Preparation time: 80
• Read length: = to fragment length
• Input amount: 1000 ng dsDNA

Short Fragment Buffer (SFB)

Flow Cell Priming Kit XL

This product has a lead time of 3 weeks

The Sequencing Auxiliary Vials contain Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS) and Loading Beads II (LBII).

This expansion is suitable for use with Kit 10, Kit 11 and Kit 12 chemistry.

The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or PromethION flow cells.

Flow Cell Priming Kit

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required.

This product has a 3 week lead time

• Preparation time: 135
• Read length: = to fragment length
• Input amount: 400 ng dsDNA