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Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Oxford Nanopore TechnologiesFully scalable, real-time DNA/RNA sequencing technology
Oxford Nanopore DiagnosticsLamPORE – rapid, low-cost, scalable detection of SARS-CoV-2
London Calling 2022
18th - 20th May
Sample prep
Sample prep

Ligation Sequencing Kit (Q20+) SQK-LSK112

A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This is an Early Access product

  • Preparation time: 60 minutes
  • Read length: = fragment length
  • Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR
$599.00

Native Barcoding Expansion (Q20+) EXP-NBD112

Additional library preparation reagents for the Native Barcoding Kit 24 and 96 (SQK-NBD112.24 and SQK-NBD112.96).

This is an Early Access product

This product has a 2 week lead time

  • Read length: = to fragment length
  • Input amount: 1000 ng dsDNA
$399.00

Flow Cell Wash Kit XL EXP-WSH004-XL

The Flow Cell Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells on a higher scale.

This product has a 6 week lead time

    $432.00

    cDNA-PCR Sequencing Kit SQK-PCS111

    Identification and quantification of full-length transcripts with highest output.

    This product has a 6 week lead time

    • Preparation time: 160 + PCR
    • Read length: Enriched for full-length cDNA during PCR
    • Input amount: 4 ng poly-A+ RNA, or 200 ng total RNA
    $599.00

    PCR-cDNA Barcoding Kit SQK-PCB111.12

    Identification and quantification of full-length transcripts with highest output, with barcodes for up to 12 samples.

    This is an Early Access product

    This product has a 6 week lead time

    • Preparation time: 160 + PCR
    • Read length: Enriched for full-length cDNA during PCR
    • Input amount: 4 ng poly-A+ RNA, or 200 ng total RNA
    $750.00

    Native Barcoding Kit 96 (Q20+) SQK-NBD112.96

    A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long reads.

    This is an Early Access product

    This product has a 2 week lead time

    • Preparation time: 140 minutes
    • Read length: = fragment length
    • Input amount: 400 ng high moleculare weight dsDNA per sample, 200 fmol (130 ng for 1 kb amplicons) per sample
    $799.00

    Native Barcoding Kit 24 (Q20+) SQK-NBD112.24

    A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long reads.

    This is an Early Access product

    This product has a 2 week lead time

    • Preparation time: 140 minutes
    • Read length: = fragment length
    • Input amount: 1000 ng high molecular weight dsDNA for <4 barcodes, 400 ng high moleculare weight dsDNA for >4 barcodes
    $699.00

    Ligation Sequencing Kit XL (Q20+) SQK-LSK112-XL

    A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long reads. Multiple samples can be processed simultaneously.

    This is an Early Access product

    This product has a 6 week lead time

    • Preparation time: 120 minutes
    • Read length: = fragment length
    • Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR
    $4,000.00

    COVID Maxi: 9216 samples C19MAXI

    A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

      $88,000.00

      COVID Midi: 2304 samples C19MIDI

      A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

        $24,216.00

        COVID Mini: 576 samples C19MINI

        A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

          $7,284.00

          Midnight RT PCR Expansion EXP-MRT001

          An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

          • Preparation time: 80 minutes + PCR
          • Read length: = to fragment length post-PCR
          $2,880.00

          Adapter Mix II Expansion EXP-AMII001

          Vials of Adapter Mix II

          This product has a 2 week lead time

            $199.00

            Ligation Sequencing Kit SQK-LSK110

            A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples, we recommend using the sequencing kits: Native Barcoding Kit 24 (SQK-NBD112.24) or the Native Barcoding Kit 96 (SQK-NBD112.96) which use our Kit 12 leading chemistry.

            • Preparation time: 60 minutes
            • Read length: = fragment length
            • Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR
            $599.00

            Flow Cell Wash Kit EXP-WSH004

            The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

              $99.00

              Ligation Sequencing Kit SQK-LSK109

              A ligation-based sequencing kit for multiplexing samples.
              For singleplex samples, please use our new version: SQK-LSK112. All COVID-related projects will be supported indefinitely. For other projects, we will discontinue the kit 3 months after the introduction of the Native Barcoding Kit 24 (SQK-NBD112.24) and the Native Barcoding Kit 96 (SQK-NBD112.96). Includes a Flow Cell Priming Kit

              • Preparation time: 60
              • Read length: = fragment length
              • Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR
              $599.00

              Rapid Sequencing Kit SQK-RAD004

              A simple and rapid library preparation method for genomic DNA.

              Includes a Flow Cell Priming Kit

              • Preparation time: 10
              • Read length: Random distribution, dependent on input fragment length
              • Input amount: 400 ng HMW gDNA
              $599.00

              PCR Sequencing Kit SQK-PSK004

              This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

              Includes a Flow Cell Priming Kit

              • Preparation time: PCR + 60
              • Read length: = fragment length post-PCR
              • Input amount: 100 ng dsDNA
              $599.00

              Rapid PCR Barcoding Kit SQK-RPB004

              Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

              Includes a Flow Cell Priming Kit

              • Preparation time: PCR + 15
              • Read length: ~2 kb
              • Input amount: 1-5 ng gDNA
              $650.00

              Ultra-Long DNA Sequencing Kit SQK-ULK001

              Ultra-Long DNA Sequencing Kit

              • Preparation time: 610 minutes
              • Read length: N50 >50 kb
              $1,200.00

              PCR Barcoding Kit SQK-PBK004

              This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR.

              Includes a Flow Cell Priming Kit

              • Preparation time: PCR + 60
              • Read length: = fragment length post-PCR
              • Input amount: 100 ng dsDNA
              $650.00

              Rapid Barcoding Kit 96 SQK-RBK110.96

              Simple and rapid library preparation, with barcoding for up to 96 gDNA samples

              This product has a one-week lead time

              • Preparation time: 60
              • Read length: Random distribution, dependent on input fragment length
              • Input amount: 50 ng HMW gDNA
              $990.00

              Rapid Barcoding Kit SQK-RBK004

              A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples.

              Includes a Flow Cell Priming Kit

              • Preparation time: 10
              • Read length: Random distribution, dependent on input fragment length
              • Input amount: 400 ng HMW gDNA
              $650.00

              Direct cDNA Sequencing Kit SQK-DCS109

              Identification and quantification of full-length transcripts without PCR bias.

              Includes a Flow Cell Priming Kit

              • Preparation time: 275
              • Read length: Enriched for full-length cDNA
              • Input amount: 100 ng poly-A+ RNA
              $599.00

              PCR-cDNA Sequencing Kit SQK-PCS109

              Identification and quantification of full-length transcripts with highest throughput

              Includes a Flow Cell Priming Kit

              • Preparation time: 165
              • Read length: Enriched for full-length cDNA
              • Input amount: 1 ng poly-A+ or 50 ng total RNA
              $599.00

              Direct RNA Sequencing Kit SQK-RNA002

              Prepare RNA for direct RNA nanopore sequencing

              Includes a Flow Cell Priming Kit

              • Preparation time: 115
              • Read length: = RNA length
              • Input amount: 500 ng RNA (poly-A+)
              $599.00

              Field Sequencing Kit SQK-LRK001

              Cold chain-free library preparation for ultimate portability.

              Includes a Flow Cell Priming Kit

              • Preparation time: 10
              • Read length: Random distribution, dependent on input fragment length
              • Input amount: 400 ng dsDNA
              $599.00

              16S Barcoding Kit 1-24 SQK-16S024

              Genus-level bacterial identification with barcoding for up to 24 samples.

              Includes a Flow Cell Priming Kit

              • Preparation time: PCR + 10
              • Read length: = full-length 16S gene (~1.5kb)
              • Input amount: 10 ng gDNA
              $900.00

              Ligation Sequencing Kit XL SQK-LSK109-XL

              A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

              • Preparation time: 130
              • Read length: = fragment length
              • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
              $4,000.00

              PCR-cDNA Barcoding Kit SQK-PCB109

              Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

              Includes a Flow Cell Priming Kit

              • Preparation time: 165
              • Read length: Enriched for full-length cDNA during PCR
              • Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA
              $650.00

              Cas9 Sequencing Kit SQK-CS9109

              Targeted sequencing of genomic regions using CRISPR-Cas9

              Includes a Flow Cell Priming Kit

              • Preparation time: 110
              • Read length: = fragment length
              • Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)
              $625.00

              16S Barcoding Kit SQK-RAB204

              This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time.

              Includes a Flow Cell Priming Kit

              • Preparation time: PCR + 10
              • Read length: = full-length 16S gene (~1.5kb)
              • Input amount: 10 ng gDNA
              $650.00

              Control Expansion EXP-CTL001

              Lambda DNA (LMD). Pack of six vials

                $60.00

                PCR Expansion EXP-PCA001

                PCR Primers (PRM) and PCR Adapters (PCA)

                  $420.00

                  RAP Top-Up Kit EXP-RAP001

                  Rapid Sequencing Adapters (RAP)

                    $420.00

                    PCR Barcoding Expansion 1-96 EXP-PBC096

                    The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode.

                    • Preparation time: 150'
                    • Read length: = to fragment length post PCR
                    • Input amount: 1000 ng dsDNA
                    $1,700.00

                    PCR Barcoding Expansion 1-12 EXP-PBC001

                    The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes, sufficient for 6 reactions.

                    • Preparation time: 150'
                    • Read length: = to fragment length post PCR
                    • Input amount: 1000 ng dsDNA
                    $288.00

                    Native Barcoding Expansion 13-24 (PCR-free) EXP-NBD114

                    A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

                    • Preparation time: 80
                    • Read length: = to fragment length
                    • Input amount: 1000 ng dsDNA
                    $288.00

                    Native Barcoding Expansion 1-12 (PCR-free) EXP-NBD104

                    A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

                    • Preparation time: 80
                    • Read length: = to fragment length
                    • Input amount: 1000 ng dsDNA
                    $288.00

                    SFB Expansion EXP-SFB001

                    Short Fragment Buffer (SFB)

                      $30.00

                      Flow Cell Priming Kit XL EXP-FLP002-XL

                      Flow Cell Priming Kit XL

                      This product has a lead time of 3 weeks

                        $180.00

                        Sequencing Auxiliary Vials EXP-AUX110

                        The Sequencing Auxiliary Vials contain Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS) and Loading Beads II (LBII).

                        This expansion is suitable for use with Kit 10, Kit 11 and Kit 12 chemistry.

                          $99.00

                          Sequencing Auxiliary Vials EXP-AUX001

                          The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or PromethION flow cells.

                            $99.00

                            Flow Cell Priming Kit EXP-FLP002

                            Flow Cell Priming Kit

                              $35.00

                              Native Barcoding Expansion 96 EXP-NBD196

                              A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required.

                              This product has a 3 week lead time

                              • Preparation time: 135
                              • Read length: = to fragment length
                              • Input amount: 400 ng dsDNA
                              $1,200.00