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Sequencing kits Expansion Packs Compare Sequencing Kits VolTRAX

Sequencing kits

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Ligation Sequencing Kit

SQK-LSK110

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109

Key features:

Prep time	60 minutes minutes
Input amount	1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
Read length	= fragment length
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Ligation Sequencing Kit XL

SQK-LSK109-XL

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

Key features:

Prep time	110 minutes
Input amount	1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
Read length	= fragment length
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$4,000.00

16S Barcoding Kit 1-24

SQK-16S024

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 10 minutes
Input amount	10 ng gDNA
Read length	= full-length 16S gene (~1.5kb)
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$900.00

Cas9 Sequencing Kit

SQK-CS9109

Targeted sequencing of genomic regions using CRISPR-Cas9

Includes a Flow Cell Priming Kit

Key features:

Prep time	110 minutes
Input amount	1–10 µg of double-stranded DNA (5 µg recommended)
Read length	= fragment length
Typical throughput	<1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION

$625.00

PCR-cDNA Barcoding Kit

SQK-PCB109

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	165 minutes
Input amount	1 ng poly-A+ RNA, or 50 ng total RNA
Read length	Enriched for full-length cDNA during PCR
Typical throughput	7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION

$650.00

Direct RNA Sequencing Kit

SQK-RNA002

Sequence RNA molecules directly and preserve base modifications

Includes a Flow Cell Priming Kit

Key features:

Prep time	115 minutes
Input amount	500 ng RNA (poly-A+)
Read length	= RNA length
Typical throughput	<1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION

$599.00

Field Sequencing Kit

SQK-LRK001

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

Key features:

Prep time	10 minutes
Input amount	400 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$599.00

PCR-cDNA Sequencing Kit

SQK-PCS109

Identification and quantification of full-length transcripts with highest throughput

Includes a Flow Cell Priming Kit

Key features:

Prep time	165 minutes
Input amount	1 ng poly-A+ or 50 ng total RNA
Read length	Enriched for full-length cDNA
Typical throughput	7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION

$599.00

Direct cDNA Sequencing Kit

SQK-DCS109

Identification and quantification of full-length transcripts without PCR bias

Includes a Flow Cell Priming Kit

Key features:

Prep time	270 minutes
Input amount	100 ng poly-A+ RNA
Read length	Enriched for full-length cDNA
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Added to basket: Direct cDNA Sequencing Kit

To barcode please select one of these kits:

Native Barcoding Expansion 1-12 (PCR-free)

EXP-NBD104

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

Native Barcoding Expansion 13-24 (PCR-free)

EXP-NBD114

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

PCR Barcoding Kit

SQK-PBK004

Control over read length or amplicon sequencing, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 60 minutes
Input amount	100 ng dsDNA
Read length	= fragment length post-PCR
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION

$650.00

Rapid Barcoding Kit

SQK-RBK004

Simple and rapid library preparation, with barcoding for up to 12 gDNA samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	10 minutes
Input amount	400 ng HMW gDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00

Rapid PCR Barcoding Kit

SQK-RPB004

Simple library preparation with barcoding for up to 12 gDNA samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 15 minutes
Input amount	1-5 ng gDNA
Read length	~2 kb
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00

PCR Sequencing Kit

SQK-PSK004

Control over read length and compatible with targeted amplicon sequencing

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 60 minutes
Input amount	100 ng dsDNA
Read length	= fragment length post-PCR
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION

$599.00

16S Barcoding Kit

SQK-RAB204

Genus level bacterial identification with barcoding for up to 12 samples.

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 10 minutes
Input amount	10 ng gDNA
Read length	= full-length 16S gene (~1.5kb)
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00

Rapid Sequencing Kit

SQK-RAD004

A simple and rapid library preparation method for genomic DNA

Includes a Flow Cell Priming Kit

Key features:

Prep time	10 minutes
Input amount	400 ng HMW gDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$599.00

Ligation Sequencing Kit

SQK-LSK109

A ligation-based sequencing kit for multiplexing samples.
For singleplex samples, please use our new version: SQK-LSK110.
All COVID-related projects will be supported indefinitely. For other projects, we will discontinue the kit on Sept 10, 2021.

Includes a Flow Cell Priming Kit

Key features:

Prep time	60 minutes
Input amount	1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
Read length	= fragment length
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Expansion packs

Native Barcoding Expansion 96

EXP-NBD196

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

$1,200.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

$1,200.00

Native Barcoding Expansion 1-12 (PCR-free)

EXP-NBD104

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

Native Barcoding Expansion 13-24 (PCR-free)

EXP-NBD114

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

PCR Barcoding Expansion 1-12

EXP-PBC001

gDNA / amplicon / cDNA ~150 mins + PCR PCR

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

$288.00

gDNA / amplicon / cDNA ~150 mins + PCR PCR

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

$288.00

Compare kits

Select the type of kits you would like to compare from the three options below.

Voltrax

VolTRAX Sequencing Kit

VSK-VSK002

A simple and rapid library preparation method for genomic DNA on the VolTRAX

Includes a Flow Cell Priming Kit

Key features:

Handle Time	10 minutes
Concentration	475 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	10 – 20 Gb in 48 hours

$149.00

VolTRAX Cartridge Pack

VCT-V2002

The VolTRAX V2 Cartridge Pack contains three disposable cartridges that interface with the VolTRAX V2 device.

Key features:

Handle Time	5 minutes
Concentration	400 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	10 - 20 GB in 48 hours

$900.00

VolTRAX Multiplex Kit

VSK-VMK002

A simple and rapid library preparation method for genomic DNA on the VolTRAX, with barcoding for up to 10 gDNA samples.

This product has a lead time of 6 weeks.

Key features:

Handle Time	10 minutes
Concentration	80 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	10 – 20 Gb in 48 hours

$220.00

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Sample Prep

Sequencing kits

Ligation Sequencing Kit

Ligation Sequencing Kit XL

16S Barcoding Kit 1-24

Cas9 Sequencing Kit

PCR-cDNA Barcoding Kit

Direct RNA Sequencing Kit

Field Sequencing Kit

PCR-cDNA Sequencing Kit

Added to basket: PCR-cDNA Sequencing Kit

PCR Barcoding Kit

Direct cDNA Sequencing Kit

Added to basket: Direct cDNA Sequencing Kit

Native Barcoding Expansion 1-12 (PCR-free)

Native Barcoding Expansion 13-24 (PCR-free)

PCR Barcoding Kit

Rapid Barcoding Kit

Rapid PCR Barcoding Kit

PCR Sequencing Kit

16S Barcoding Kit

Rapid Sequencing Kit

Ligation Sequencing Kit

Added to basket: Ligation Sequencing Kit

Native Barcoding Expansion 1-12 (PCR-free)

Native Barcoding Expansion 13-24 (PCR-free)

PCR Barcoding Expansion 1-12

PCR Barcoding Expansion 1-96

Expansion packs

Flow Cell Priming Kit

Sequencing Auxiliary Vials

Flow Cell Priming Kit XL

SFB Expansion

Native Barcoding Expansion 96

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