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Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Sample Prep

Sequencing kits

Compare

Ligation Sequencing Kit

SQK-LSK110

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109

Key features:

Prep time

60 minutes minutes

Input amount

1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Read length

= fragment length

Typical throughput

2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00
Ligation Sequencing Kit XL

SQK-LSK109-XL

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

Key features:

Prep time

110 minutes

Input amount

1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Read length

= fragment length

Typical throughput

2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$4,000.00
16S Barcoding Kit 1-24

SQK-16S024

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit

Key features:

Prep time

PCR + 10 minutes

Input amount

10 ng gDNA

Read length

= full-length 16S gene (~1.5kb)

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$900.00
Cas9 Sequencing Kit

SQK-CS9109

Targeted sequencing of genomic regions using CRISPR-Cas9

Includes a Flow Cell Priming Kit

Key features:

Prep time

110 minutes

Input amount

1–10 µg of double-stranded DNA (5 µg recommended)

Read length

= fragment length

Typical throughput

<1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION

$625.00
PCR-cDNA Barcoding Kit

SQK-PCB109

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

Key features:

Prep time

165 minutes

Input amount

1 ng poly-A+ RNA, or 50 ng total RNA

Read length

Enriched for full-length cDNA during PCR

Typical throughput

7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION

$650.00
Direct RNA Sequencing Kit

SQK-RNA002

Sequence RNA molecules directly and preserve base modifications

Includes a Flow Cell Priming Kit

Key features:

Prep time

115 minutes

Input amount

500 ng RNA (poly-A+)

Read length

= RNA length

Typical throughput

<1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION

$599.00
Field Sequencing Kit

SQK-LRK001

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

Key features:

Prep time

10 minutes

Input amount

400 ng dsDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$599.00
PCR-cDNA Sequencing Kit

SQK-PCS109

Identification and quantification of full-length transcripts with highest throughput

Includes a Flow Cell Priming Kit

Key features:

Prep time

165 minutes

Input amount

1 ng poly-A+ or 50 ng total RNA

Read length

Enriched for full-length cDNA

Typical throughput

7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION

$599.00
Direct cDNA Sequencing Kit

SQK-DCS109

Identification and quantification of full-length transcripts without PCR bias

Includes a Flow Cell Priming Kit

Key features:

Prep time

270 minutes

Input amount

100 ng poly-A+ RNA

Read length

Enriched for full-length cDNA

Typical throughput

2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00
PCR Barcoding Kit

SQK-PBK004

Control over read length or amplicon sequencing, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

Key features:

Prep time

PCR + 60 minutes

Input amount

100 ng dsDNA

Read length

= fragment length post-PCR

Typical throughput

2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION

$650.00
Rapid Barcoding Kit

SQK-RBK004

Simple and rapid library preparation, with barcoding for up to 12 gDNA samples

Includes a Flow Cell Priming Kit

Key features:

Prep time

10 minutes

Input amount

400 ng HMW gDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00
Rapid PCR Barcoding Kit

SQK-RPB004

Simple library preparation with barcoding for up to 12 gDNA samples

Includes a Flow Cell Priming Kit

Key features:

Prep time

PCR + 15 minutes

Input amount

1-5 ng gDNA

Read length

~2 kb

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00
PCR Sequencing Kit

SQK-PSK004

Control over read length and compatible with targeted amplicon sequencing

Includes a Flow Cell Priming Kit

Key features:

Prep time

PCR + 60 minutes

Input amount

100 ng dsDNA

Read length

= fragment length post-PCR

Typical throughput

2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION

$599.00
16S Barcoding Kit

SQK-RAB204

Genus level bacterial identification with barcoding for up to 12 samples.

Includes a Flow Cell Priming Kit

Key features:

Prep time

PCR + 10 minutes

Input amount

10 ng gDNA

Read length

= full-length 16S gene (~1.5kb)

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00
Rapid Sequencing Kit

SQK-RAD004

A simple and rapid library preparation method for genomic DNA 

Includes a Flow Cell Priming Kit

Key features:

Prep time

10 minutes

Input amount

400 ng HMW gDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$599.00
Ligation Sequencing Kit

SQK-LSK109

A ligation-based sequencing kit for multiplexing samples.  
For singleplex samples, please use our new version: SQK-LSK110.
All COVID-related projects will be supported indefinitely. For other projects, we will discontinue the kit on Sept 10, 2021.

Includes a Flow Cell Priming Kit

Key features:

Prep time

60 minutes

Input amount

1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Read length

= fragment length

Typical throughput

2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Expansion packs

Flow Cell Priming Kit

EXP-FLP002

Flow Cell Priming Kit

$35.00

Flow Cell Priming Kit

$35.00
Sequencing Auxiliary Vials

EXP-AUX001

The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or Promet...

$99.00

The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or Promet...

$99.00
Flow Cell Priming Kit XL

EXP-FLP002-XL

Flow Cell Priming Kit XL This product has a lead time of 3 weeks.

$180.00

Flow Cell Priming Kit XL This product has a lead time of 3 weeks.

$180.00
SFB Expansion

EXP-SFB001

Short Fragment Buffer (SFB)

$30.00

Short Fragment Buffer (SFB)

$30.00
Native Barcoding Expansion 96

EXP-NBD196

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

$1,200.00
gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

$1,200.00
Sequencing Auxiliary Vials

EXP-AUX110

Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS) and Loading Beads II (LBII).

$99.00

Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS) and Loading Beads II (LBII).

$99.00
Flow Cell Wash Kit

EXP-WSH004

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

$85.00

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

$85.00
Native Barcoding Expansion 1-12 (PCR-free)

EXP-NBD104

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00
gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00
Native Barcoding Expansion 13-24 (PCR-free)

EXP-NBD114

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00
gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00
PCR Barcoding Expansion 1-12

EXP-PBC001

gDNA / amplicon / cDNA ~150 mins + PCR PCR

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

$288.00
gDNA / amplicon / cDNA ~150 mins + PCR PCR

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

$288.00
PCR Barcoding Expansion 1-96

EXP-PBC096

gDNA / amplicon / cDNA ~150 mins + PCR PCR

Designed to allow the pooling and running together of multiple sequencing libraries

$1,700.00
gDNA / amplicon / cDNA ~150 mins + PCR PCR

Designed to allow the pooling and running together of multiple sequencing libraries

$1,700.00
RAP Top-Up Kit

EXP-RAP001

Rapid 1D sequencing adapters (RAP)

$420.00

Rapid 1D sequencing adapters (RAP)

$420.00
PCR Expansion

EXP-PCA001

PCR Primers (PRM) and PCR Adapters (PCA)

$420.00

PCR Primers (PRM) and PCR Adapters (PCA)

$420.00
Control Expansion

EXP-CTL001

Lambda DNA (LMD). Pack of six vials

$60.00

Lambda DNA (LMD). Pack of six vials

$60.00

Compare kits

Select the type of kits you would like to compare from the three options below.

PCR-free Kits

PCR Kits

RNA Kits

Voltrax

VolTRAX Sequencing Kit

VSK-VSK002

A simple and rapid library preparation method for genomic DNA on the VolTRAX

Includes a Flow Cell Priming Kit

Key features:

Handle Time

10 minutes

Concentration

475 ng dsDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

10 – 20 Gb in 48 hours

$149.00
VolTRAX Cartridge Pack

VCT-V2002

The VolTRAX V2 Cartridge Pack contains three disposable cartridges that interface with the VolTRAX V2 device.

Key features:

Handle Time

5 minutes

Concentration

400 ng dsDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

10 - 20 GB in 48 hours

$900.00
VolTRAX Multiplex Kit

VSK-VMK002

A simple and rapid library preparation method for genomic DNA on the VolTRAX, with barcoding for up to 10 gDNA samples.

This product has a lead time of 6 weeks.

Key features:

Handle Time

10 minutes

Concentration

80 ng dsDNA

Read length

Random distribution, dependent on input fragment length

Typical throughput

10 – 20 Gb in 48 hours

$220.00
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