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Cas9 Sequencing Kit

SQK-CS9109

Targeted sequencing of genomic regions using CRISPR-Cas9

Key features:

Prep time	110 minutes
Input amount	1–10 µg of double-stranded DNA (5 µg recommended)
Read length	= fragment length
Typical throughput	<1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION

$625.00

PCR-cDNA Barcoding Kit

SQK-PCB109

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	165 minutes
Input amount	1 ng poly-A+ RNA, or 50 ng total RNA
Read length	Enriched for full-length cDNA during PCR
Typical throughput	7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION

$650.00

Ligation Sequencing Kit

SQK-LSK109

A ligation-based sequencing kit for multiplexing samples.
For singleplex samples, please use our new version: SQK-LSK110.
SQK-LSK109 is to be discontinued on May 10, 2021.

Includes a Flow Cell Priming Kit

Key features:

Prep time	60 minutes
Input amount	1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
Read length	= fragment length
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Ligation Sequencing Kit XL

SQK-LSK109-XL

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

Key features:

Prep time	110 minutes
Input amount	1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
Read length	= fragment length
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$4,000.00

16S Barcoding Kit 1-24

SQK-16S024

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 10 minutes
Input amount	10 ng gDNA
Read length	= full-length 16S gene (~1.5kb)
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$900.00

Ligation Sequencing Kit

SQK-LSK110

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109

Key features:

Prep time	60 minutes minutes
Input amount	1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR
Read length	= fragment length
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Direct RNA Sequencing Kit

SQK-RNA002

Sequence RNA molecules directly and preserve base modifications

Includes a Flow Cell Priming Kit

Key features:

Prep time	115 minutes
Input amount	500 ng RNA (poly-A+)
Read length	= RNA length
Typical throughput	<1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION

$599.00

Field Sequencing Kit

SQK-LRK001

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

Key features:

Prep time	10 minutes
Input amount	400 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$599.00

PCR-cDNA Sequencing Kit

SQK-PCS109

Identification and quantification of full-length transcripts with highest throughput

Includes a Flow Cell Priming Kit

Key features:

Prep time	165 minutes
Input amount	1 ng poly-A+ or 50 ng total RNA
Read length	Enriched for full-length cDNA
Typical throughput	7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION

$599.00

Direct cDNA Sequencing Kit

SQK-DCS109

Identification and quantification of full-length transcripts without PCR bias

Includes a Flow Cell Priming Kit

Key features:

Prep time	270 minutes
Input amount	100 ng poly-A+ RNA
Read length	Enriched for full-length cDNA
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION

$599.00

Added to basket: Direct cDNA Sequencing Kit

To barcode please select one of these kits:

Native Barcoding Expansion 1-12 (PCR-free)

EXP-NBD104

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

Native Barcoding Expansion 13-24 (PCR-free)

EXP-NBD114

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

PCR Barcoding Kit

SQK-PBK004

Control over read length or amplicon sequencing, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 60 minutes
Input amount	100 ng dsDNA
Read length	= fragment length post-PCR
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION

$650.00

Rapid Barcoding Kit

SQK-RBK004

Simple and rapid library preparation, with barcoding for up to 12 gDNA samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	10 minutes
Input amount	400 ng HMW gDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00

PCR Sequencing Kit

SQK-PSK004

Control over read length and compatible with targeted amplicon sequencing

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 60 minutes
Input amount	100 ng dsDNA
Read length	= fragment length post-PCR
Typical throughput	2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION

$599.00

16S Barcoding Kit

SQK-RAB204

Genus level bacterial identification with barcoding for up to 12 samples.

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 10 minutes
Input amount	10 ng gDNA
Read length	= full-length 16S gene (~1.5kb)
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00

Rapid PCR Barcoding Kit

SQK-RPB004

Simple library preparation with barcoding for up to 12 gDNA samples

Includes a Flow Cell Priming Kit

Key features:

Prep time	PCR + 15 minutes
Input amount	1-5 ng gDNA
Read length	~2 kb
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$650.00

Rapid Sequencing Kit

SQK-RAD004

A simple and rapid library preparation method for genomic DNA

Includes a Flow Cell Priming Kit

Key features:

Prep time	10 minutes
Input amount	400 ng HMW gDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION

$599.00

Expansion packs

Native Barcoding Expansion 96

EXP-NBD196

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

$1,200.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

$1,200.00

Native Barcoding Expansion 1-12 (PCR-free)

EXP-NBD104

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

Native Barcoding Expansion 13-24 (PCR-free)

EXP-NBD114

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

gDNA / amplicon / cDNA ≤ 1 µg ~80 mins No PCR Throughput / read-length control

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

$288.00

PCR Barcoding Expansion 1-12

EXP-PBC001

gDNA / amplicon / cDNA ~150 mins + PCR PCR

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

$288.00

gDNA / amplicon / cDNA ~150 mins + PCR PCR

The kit is designed to allow the pooling and running together of multiple sequencing libraries on the Oxford Nanopore MinION

$288.00

Compare kits

Select the type of kits you would like to compare from the three options below.

Voltrax

VolTRAX Sequencing Kit

VSK-VSK002

A simple and rapid library preparation method for genomic DNA on the VolTRAX

Includes a Flow Cell Priming Kit

Key features:

Handle Time	10 minutes
Concentration	475 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	10 – 20 Gb in 48 hours

$149.00

VolTRAX Cartridge Pack

VCT-V2002

The VolTRAX V2 Cartridge Pack contains three disposable cartridges that interface with the VolTRAX V2 device.

Key features:

Handle Time	5 minutes
Concentration	400 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	10 - 20 GB in 48 hours

$990.00

VolTRAX Multiplex Kit

VSK-VMK002

A simple and rapid library preparation method for genomic DNA on the VolTRAX, with barcoding for up to 10 gDNA samples.

This product has a lead time of 6 weeks.

Key features:

Handle Time	10 minutes
Concentration	80 ng dsDNA
Read length	Random distribution, dependent on input fragment length
Typical throughput	10 – 20 Gb in 48 hours

$179.00

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Sample Prep

Sequencing kits

Cas9 Sequencing Kit

PCR-cDNA Barcoding Kit

Ligation Sequencing Kit

Added to basket: Ligation Sequencing Kit

Native Barcoding Expansion 1-12 (PCR-free)

Native Barcoding Expansion 13-24 (PCR-free)

PCR Barcoding Expansion 1-12

PCR Barcoding Expansion 1-96

Ligation Sequencing Kit XL

16S Barcoding Kit 1-24

Ligation Sequencing Kit

Direct RNA Sequencing Kit

Field Sequencing Kit

PCR-cDNA Sequencing Kit

Added to basket: PCR-cDNA Sequencing Kit

PCR Barcoding Kit

Direct cDNA Sequencing Kit

Added to basket: Direct cDNA Sequencing Kit

Native Barcoding Expansion 1-12 (PCR-free)

Native Barcoding Expansion 13-24 (PCR-free)

PCR Barcoding Kit

Rapid Barcoding Kit

PCR Sequencing Kit

16S Barcoding Kit

Rapid PCR Barcoding Kit

Rapid Sequencing Kit

Expansion packs

Native Barcoding Expansion 96

Flow Cell Priming Kit

Sequencing Auxiliary Vials