A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This is an Early Access product

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

Identification and quantification of full-length transcripts with highest output.

This product has a 6 week lead time

• Preparation time: 160 + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly-A+ RNA, or 200 ng total RNA

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 12 samples.

This is an Early Access product

This product has a 6 week lead time

• Preparation time: 160 + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly-A+ RNA, or 200 ng total RNA

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long reads.

This is an Early Access product

This product has a 2 week lead time

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400 ng high moleculare weight dsDNA per sample, 200 fmol (130 ng for 1 kb amplicons) per sample

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long reads.

This is an Early Access product

This product has a 2 week lead time

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA for <4 barcodes, 400 ng high moleculare weight dsDNA for >4 barcodes

A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long reads. Multiple samples can be processed simultaneously.

This is an Early Access product

This product has a 6 week lead time

• Preparation time: 120 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples, we recommend using the sequencing kits: Native Barcoding Kit 24 (SQK-NBD112.24) or the Native Barcoding Kit 96 (SQK-NBD112.96) which use our Kit 12 leading chemistry.

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

A ligation-based sequencing kit for multiplexing samples.
For singleplex samples, please use our new version: SQK-LSK112. All COVID-related projects will be supported indefinitely. For other projects, we will discontinue the kit 3 months after the introduction of the Native Barcoding Kit 24 (SQK-NBD112.24) and the Native Barcoding Kit 96 (SQK-NBD112.96). Includes a Flow Cell Priming Kit

• Preparation time: 60
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple and rapid library preparation method for genomic DNA.

Includes a Flow Cell Priming Kit

• Preparation time: 10
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 60
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 15
• Read length: ~2 kb
• Input amount: 1-5 ng gDNA

Ultra-Long DNA Sequencing Kit

• Preparation time: 610 minutes
• Read length: N50 >50 kb

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 60
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples

This product has a one-week lead time

• Preparation time: 60
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng HMW gDNA

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples.

Includes a Flow Cell Priming Kit

• Preparation time: 10
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

Identification and quantification of full-length transcripts without PCR bias.

Includes a Flow Cell Priming Kit

• Preparation time: 275
• Read length: Enriched for full-length cDNA
• Input amount: 100 ng poly-A+ RNA

Identification and quantification of full-length transcripts with highest throughput

Includes a Flow Cell Priming Kit

• Preparation time: 165
• Read length: Enriched for full-length cDNA
• Input amount: 1 ng poly-A+ or 50 ng total RNA

Prepare RNA for direct RNA nanopore sequencing

Includes a Flow Cell Priming Kit

• Preparation time: 115
• Read length: = RNA length
• Input amount: 500 ng RNA (poly-A+)

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

• Preparation time: 10
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 10
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

• Preparation time: 130
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

Includes a Flow Cell Priming Kit

• Preparation time: 165
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA

Targeted sequencing of genomic regions using CRISPR-Cas9

Includes a Flow Cell Priming Kit

• Preparation time: 110
• Read length: = fragment length
• Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time.

Includes a Flow Cell Priming Kit

• Preparation time: PCR + 10
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA