An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

This is an Early Access product

Product lead time: 6 weeks

This uses our latest Kit 14 chemistry

• Preparation time: ~60 minutes + overnight elution
• Read length: N50 >50 kb
• Input amount: 6 million cells

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.

This is an Early Access product

Product lead time: 5 weeks

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

Simple and rapid library preparation, with barcoding for up to 24 gDNA samples.

This is an Early Access product

Product lead time: 4 weeks

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy

This is an Early Access product

Product lead time: 6 weeks

This uses our latest Kit 14 chemistry

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 100 ng dsDNA

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This is an Early Access product

Product lead time: 4 weeks

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA for <4 barcodes, 400 ng high moleculare weight dsDNA for >4 barcodes

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This is an Early Access product

Product lead time: 2 weeks

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400-1000 ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.

This is an Early Access product

Product lead time: 1 week

This uses our latest Kit 14 chemistry

• Preparation time: 120 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of  amplicons or cDNA

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This is an Early Access product

Product lead time: 1 week

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR,100-200 fmol of  amplicons or cDNA

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 24 samples.

This is an Early Access product

• Preparation time: 160 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA per sample

A sequencing kit optimised for output and PCR-free sequencing of 2-3 samples for Whole Genome Sequencing (WGS).

This is an Early Access product

• Preparation time: 135 minutes
• Read length: = Fragment length
• Input amount: 1000 ng dsDNA per sample

Identification and quantification of full-length transcripts with highest output.

• Preparation time: 160 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

3 week lead time

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

3 week lead time

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

3 week lead time

A versatile sequencing kit optimised for output, and long reads, and processing singleplex samples. For multiplexing samples, we recommend using the sequencing kits: Native Barcoding Kit 24 V14 (SQK-NBD114.24) or the Native Barcoding Kit 96 V14 (SQK-NBD114.96) which use our latest Kit 14 chemistry.

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR

A ligation-based sequencing kit for processing singleplex samples. We recommend using our latest version: SQK-LSK114. All COVID-related projects will be supported indefinitely.

Includes a Flow Cell Priming Kit

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple and rapid library preparation method for genomic DNA.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: 60 minutes + PCR
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: 15 minutes + PCR
• Read length: ~2 kb
• Input amount: 1-5 ng dsDNA per sample

Ultra-Long DNA Sequencing Kit

• Preparation time: 610 minutes
• Read length: N50 >50 kb
• Input amount: 6 million cells

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR.

Includes a Flow Cell Priming Kit

• Preparation time: 60 minutes + PCR
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA per sample

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample per sample

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA per sample

Identification and quantification of full-length transcripts without PCR bias.

Includes a Flow Cell Priming Kit

• Preparation time: 275 minutes
• Read length: Enriched for full-length cDNA
• Input amount: 100 ng poly(A)+ RNA

Prepare RNA for direct RNA nanopore sequencing

Includes a Flow Cell Priming Kit

• Preparation time: 115 minutes
• Read length: = RNA length
• Input amount: 500 ng total RNA, 50 ng poly(A)+ RNA

Cold chain-free library preparation for ultimate portability.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

Genus-level bacterial identification with barcoding for up to 24 samples.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes + PCR
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng dsDNA per sample

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

• Preparation time: 130 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR

Targeted sequencing of genomic regions using CRISPR-Cas9

Includes a Flow Cell Priming Kit

• Preparation time: 110 minutes
• Read length: = fragment length
• Input amount: 1–10 µg of dsDNA

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes + PCR
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng dsDNA per sample